6533b82afe1ef96bd128b91a

RESEARCH PRODUCT

Monitoring of Cell Layer Integrity with a Current-Driven Organic Electrochemical Transistor

Paschalis GkoupidenisPaul W. M. BlomVolker MailänderVolker MailänderMatteo GhittorelliJonas ReinholzJonas ReinholzFabrizio TorricelliN. Irina CrăciunMaximilian BrücknerMaximilian BrücknerLeona V. Lingstedt

subject

Materials scienceTransistors Electroniccell barriersBiomedical EngineeringPharmaceutical ScienceBiosensing Techniques02 engineering and technologybioelectronics010402 general chemistry01 natural scienceslaw.inventionBiomaterialslawElectrochemistryHumansTransient responseinvertersCell ShapeIon transporterBioelectronicsTight junctionbioelectronics; cell barriers; inverters; organic electrochemical transistors; toxicologybusiness.industryTransistorHydrogen Peroxide021001 nanoscience & nanotechnologyorganic electrochemical transistors0104 chemical sciencesParacellular transportOptoelectronicsCaco-2 Cells0210 nano-technologybusinesstoxicologyVoltageOrganic electrochemical transistor

description

Abstract The integrity of CaCo-2 cell barriers is investigated by organic electrochemical transistors (OECTs) in a current-driven configuration. Ion transport through cellular barriers via the paracellular pathway is modulated by tight junctions between adjacent cells. Rupturing its integrity by H2O2 is monitored by the change of the output voltage in the transfer characteristics. It is demonstrated that by operating the OECT in a current-driven configuration, the sensitive and temporal resolution for monitoring the cell barrier integrity is strongly enhanced as compared to the OECT transient response measurement. As a result, current-driven OECTs are useful tools to assess dynamic and critical changes in tight junctions, relevant for clinical applications as drug targeting and screening.

yearjournalcountryeditionlanguage
2019-01-01
10.1002/adhm.201900128https://hdl.handle.net/21.11116/0000-000A-F44F-B21.11116/0000-0004-5B38-8