Mapping and determinism of soil microbial community distribution across an agricultural landscape.

Article en open access; International audience; Despite the relevance of landscape, regarding the spatial patterning of microbial communities and the relative influence of environmental parameters versus human activities, few investigations have been conducted at this scale. Here, we used a systematic grid to characterize the distribution of soil microbial communities at 278 sites across a monitored agricultural landscape of 13km(2). Molecular microbial biomass was estimated by soil DNA recovery and bacterial diversity by 16S rRNA gene pyrosequencing. Geostatistics provided the first maps of microbial community at this scale and revealed a heterogeneous but spatially structured distribution…

Diversité des communautés microbiennes telluriques à l'échelle du territoire national

Communication orale - résumé Session 5 : sols, biodiversité, écologie fontionnelleCommunication orale - résumé Session 5 : sols, biodiversité, écologie fontionnelle; absent

Biogeographical patterns of soil molecular microbial biomass as influenced by soil characteristics and management

Aim The spatial organization of soil microbial communities on large scales and the identification of environmental factors structuring their distribution have been little investigated. The overall objective of this study was to determine the spatial patterning of microbial biomass in soils over a wide extent and to rank the environmental filters most influencing this distribution.

Bacterial social networks across the French National territory

EA SPE BIOME GENOSOL UB Agrosup EA SPE BIOME GENOSOL UB AgrosupEASPEBIOMEGENOSOL UB Agrosup; New generation sequencing evidenced that soil houses a huge microbial biodiversity. Based on these observations, it is now possible to better describe the spatial distribution of soil microbial communities, to study the ecological processes involved (environmental selection, dispersal limitations), and to compute the data to define the ecological attributes of soil microbial taxa. These huge datasets also offer the opportunity to study how soil microbial populations are interrelated. With this study, we revealed the first soil bacterial "social" network across French national territory based on 2,20…

Biogeographical design of soil microbial habitats

International audience; Since the beginning of 21th century, soil microbial diversity is intensively studied gathering ahuge knowledge at local and global scales. Nevertheless, the microbial habitats have beenpoorly investigated, limiting our ability to link biodiversity description and regulation. In our study,we used a pyrosequencing approach targeting 16S rRNA genes directly amplified from soil DNAto have a comprehensive view of soil bacterial and archaeal community composition across thelargest spatially explicit soil sampling available in France (2173 soils, area covered = 5.5x105km2). Based on the multivariate regression tree (MRT) method, we designed 16 distinctterrestrial microbial …

Bacterial "social" network in french soils : a Metagenomics insight

EA SPE BIOME GENOSOL UB Agrosup EA SPE BIOME GENOSOL UB AgrosupEASPEBIOMEGENOSOL UB Agrosup; New generation sequencing evidenced that soil houses a huge microbial biodiversity. Based on these observations, it is now possible to better describe the spatial distribution of soil microbial communities, to study the ecological processes lying behind (environmental selection, dispersal limitations), and to mine the data to define the ecological attributes of soil microbial taxa. These huge datasets also offer the opportunity to study how soil microbial populations are interrelated. The objective of this study was to design the first bacterial "social" network in french soils to characterize how b…

Le projet ECOMIC-RMQS ou comment faire de la microbiogéographie à l'échelle de la France

Biogeography of soil microbial communities: a review and a description of the ongoing french national initiative

International audience; Microbial biogeography is the study of the distribution of microbial diversity on large scales of space and time. This science aims at understanding biodiversity regulation and its link with ecosystem biological functioning, goods and services such as maintenance of productivity, of soil and atmospheric quality, and of soil health. Although the initial concept dates from the early 20th century (Beijerinck (1913) De infusies en de ontdekking der backterien, in: Jaarboek van de Knoniklijke Akademie van Wetenschappen, Muller, Amsterdam), only recently have an increasing number of studies have investigated the biogeographical patterns of soil microbial diversity. A such …

Is soil an island for bacteria ? Contribution of the soil bacterial taxa area relationship unsing new generation sequencing

EA SPE BIOME GENOSOL; International audience

Spatialisation multi-échelle des émissions de N2O par les sols et de leur potentiel d’abattement en Région Bourgogne Franche-Comté : intérêts, limites et pistes de valorisation pour l’aide à la décision dans le cadre de la transition écologique aux échelles loco-régionales

Factors influencing spatial patterns of activity of denitrifying bacteria in a grassland soil

International audience

Mapping field-scale spatial patterns of size and activity of the denitrifier community

International audience; There is ample evidence that microbial processes can exhibit large variations in activity on a field scale. However, very little is known about the spatial distribution of the microbial communities mediating these processes. Here we used geostatistical modelling to explore spatial patterns of size and activity of the denitrifying community, a functional guild involved in N-cycling, in a grassland field subjected to different cattle grazing regimes. We observed a non-random distribution pattern of the size of the denitrifier community estimated by quantification of the denitrification genes copy numbers with a macro-scale spatial dependence (6–16 m) and mapped the dis…

Mapping spatial patterns of denitrifiers for bridging community ecology and microbial processes along environmental gradients

International audience; While there is ample evidence that microbial processes can exhibit large variations at a field scale, very little is known about the spatial distribution of the communities mediating these processes. The objective of this study was to explore spatial patterns of size and activity of the denitrifying community, a functional guild involved in N-cycling, in a grassland field subjected to different cattle grazing regimes. We used geostatistical modeling to map the distribution of size and activity of the denitrifier community in the pasture. Size of the denitrifier community was estimated by PCR quantification of the denitrification gene copy numbers while its activity w…

Le séquençage massif pour caractériser la diversité microbienne des sols, standardisation et applications au sein de la plateforme GenoSol

EA SPE GenoSol EcolDur; National audience; Les microorganismes (bactéries, archées et champignons) sont les organismes les plus abondants et les plus diversifiés de la matrice sol. Ils représentent un réservoir encore largement méconnu et presque infini de ressources génétiques, fortement impliqué dans le fonctionnement biologique du sol et plus largement dans la plupart des services écosystémiques fournis par ce sol. Malgré ce rôle central dans le fonctionnement des écosystèmes, nos connaissances sur la diversité génétique, taxonomique et fonctionnelle de ces microorganismes restent limitées à ce jour. Ceci s’explique essentiellement par des difficultés techniques pour accéder à ces organi…

Biogeographical patterns of soil bacterial communities.

International audience; This study provides the first maps of variations in bacterial community structure on a broad scale based on genotyping of DNA extracts from 593 soils from four different regions of France (North, Brittany, South-East and Landes). Soils were obtained from the soil library of RMQS (Réseau de Mesures de la Qualité des Sols = French soil quality monitoring network). The relevance of a biogeographic approach for studying bacterial communities was demonstrated by the great variability in community structure and specific geographical patterns within and between the four regions. The data indicated that the distribution of bacterial community composition might be more relate…

Exploring the links between Vis NIR spectra and bacterial communities structure of soils by PLS2 regression models

International audience

Influence du type de sol et du mode d’occupation des sols sur la quantité d’ADN extraite et la densité des communautés bactériennes indigènes

National audience

Determinants of the distribution of nitrogen-cycling microbial communities at the landscape-scale

Little information is available regarding the landscape-scale distribution of microbial communities and its environmental determinants. However, a landscape perspective is needed to understand the relative importance of local and regional factors and land management for the microbial communities. In this manuscript, we investigated the distribution of functional microbial communities involved in N-cycling and of the total bacterial and crenarchaeal communities over 107 sites using a grid with a 16 km lag distance within Burgundy, a 31 500 km2 region in France. After quantifying the abundances of the total bacterial, crenarchaeal, nitrate-reducing, denitrifying and ammonia-oxidizing communit…

Mapping field-scale spatial distribution patterns of size and activity of the denitrifier community

International audience

Distribution et déterminisme de la diversité des communautés de champignons telluriques à l'échelle de trois régions françaises

aeres : C-ACTN; National audience; no abstract

Spatial patterns of bacterial taxa in nature reflect ecological traits of deep branches of the 16S rRNA bacterial tree

International audience; Whether bacteria display spatial patterns of distribution and at which level of taxonomic organization such patterns can be observed are central questions in microbial ecology. Here we investigated how the total and relative abundances of eight bacterial taxa at the phylum or class level were spatially distributed in a pasture by using quantitative PCR and geostatistical modelling. The distributions of the relative abundance of most taxa varied by a factor of 2.5–6.5 and displayed strong spatial patterns at the field scale. These spatial patterns were taxon-specific and correlated to soil properties, which indicates that members of a bacterial clade defined at high t…

Contrasting Spatial Patterns and Ecological Attributes of Soil Bacterial Taxa across French National Territory

EA SPE BIOME GENOSOL UB UB Agrosup EA SPE BIOME GENOSOL UB UB AgrosupEASPEBIOMEGENOSOLUB UB Agrosup; Even though recent studies have clarified the influence of environmental filters on bacterial community diversity, those constraining bacterial populations variations remain unclear. In consequence, our ability to understand the ecological attributes of soil bacteria and to predict microbial community response to environmental stress is therefore limited. Here, we characterized the bacterial community composition and the various bacterial taxonomic groups constituting the community across the French country, by using a 16x16 km systematic grid representing 1825 sites to precisely decipher th…

Biogeographical patterns of soil bacterial diversity at the scale of France

EA SPE BIOME GENOSOLEASPEBIOMEGENOSOL; Biogeographical patterns of soil bacterial diversity at the scale of France. 16. International Symposium on Microbial Ecology - ISME16

Ecomic - RMQs : Cartographie de la diversité microbienne des sols à l'échelle de la France

aeres : C-AFF; National audience; no abstract

Mapping spatial patterns of denitrifiers for bridging community ecology and microbial processes along environmental gradients

While there is ample evidence that microbial processes can exhibit large variations at a field scale, very little is known about the spatial distribution of the communities mediating these processes. The objective of this study was to explore spatial patterns of size and activity of the denitrifying community, a functional guild involved in N-cycling, in a grassland field subjected to different cattle grazing regimes. We used geostatistical modeling to map the distribution of size and activity of the denitrifier community in the pasture. Size of the denitrifier community was estimated by PCR quantification of the denitrification gene copy numbers while its activity was estimated by measurin…