0000000000672600

AUTHOR

A. Aquino

showing 2 related works from this author

A new form of tumor and fetal collagen that binds laminin.

1993

Human breast and colon carcinoma tissues contain a form of collagen, not described before, composed of alpha 1 chains of similar size (approximately 100 kDa) but different charge. The three constitutive chains, separated by two-dimensional electrophoresis, are a unique acidic component, undetectable in other collagen types, with an apparent isoelectric point of 4-5, and two more basic components displaying the same electrophoretic behavior as alpha 1(III) and alpha 1(I), respectively. The acidic chain is structurally distinct from alpha 1(I) and displays a cyanogen bromide-derived fragment of similar size to CB5(III). This collagen in its native state is resistant to trypsin and metalloprot…

Breast NeoplasmsBiologyBiochemistryUmbilical Cordchemistry.chemical_compoundFetusLamininmedicineElectrochemistryAnimalsHumansTrypsinCyanogen BromideIsoelectric PointPolyacrylamide gel electrophoresisSkinchemistry.chemical_classificationMetalloproteinaseMetalloendopeptidasesTrypsinMolecular biologyPeptide FragmentsIntestinesMicroscopy ElectronIsoelectric pointchemistryImmunologyColonic Neoplasmsbiology.proteinCyanogen bromideCattleElectrophoresis Polyacrylamide GelCollagenLamininProtein AGlycoproteinmedicine.drugBiochemistry
researchProduct

Onco-fetal/laminin-binding collagen from colon carcinoma: detection of new sequences.

1995

We have recently identified an oncofetal-laminin binding collagen (OF/LB) composed of three alpha chains, with the apparent molecular mass of about 100 kDa each, but bearing different pI. One of the chains appears markedly acidic in a bidimensional electrophoretic system, where the NEPHGE is used as first dimension separating gel, while the two more basic chains have similar migration as alpha 1(III) and alpha 1(I) collagen chains, respectively. Sequence analyses have been performed on CNBr-peptides, derived from pepsinized triple helical molecules and on tryptic fragments obtained after in gel digestion of the acidic band. The research of sequence homology with computerized databases indic…

Macromolecular SubstancesBiopsyMolecular Sequence DataBiophysicsSequence (biology)In-gel digestionBiochemistryMoleculeHumansElectrophoresis Gel Two-DimensionalTrypsinAmino Acid SequenceCyanogen BromideLaminin bindingMolecular BiologyGeneChromatography High Pressure LiquidFetusMolecular massSequence Homology Amino AcidChemistryCell BiologyMolecular biologyPeptide FragmentsElectrophoresisBiochemistryColonic NeoplasmsCollagenBiochemical and biophysical research communications
researchProduct