0000000000679867
AUTHOR
F Bonanno
Dual Boundary Element Method for fatigue crack growth: implementation of the Richard’s criterion
A new criterion for fatigue crack growth, whose accuracy was previously tested in the literature with the Finite Element Method, is here adopted with a Dual Boundary Element formulation. The fatigue crack growth of an elliptical inclined crack, embedded in a three dimensional cylindrical bar, is analyzed. In this way in addition to the propagation angle estimated by the Sih’s criterion, it is possible to take into account a twist propagation angle. The two propagation criteria are compared in terms of shape of the propagated crack and in terms of SIFs along the crack front. The efficiency of the Dual Boundary Element Method in this study is highlighted.
P. lividus oogenesis and early development require autophagy
It is well known that autophagy is a major intracellular pathway for the degradation and recycling of cytosolic components, in both basal and stress conditions. We have recently demonstrated the activation of autophagy in P. lividus embryos: at high levels, after a stress induced by cadmium, and at basal levels, during the physiological development (1) (2). Here we report ours recent data about autophagy during oogenesis and segmentation. In order to detect autophagolysosomes (AVOs) and autolysosomes, we respectively performed incubation in vivo with acridine orange (AO), and in situ immunofluorescence (IF) with the anti-LC3 antibody (autophagy marker). All observations were carried out by …