0000000000683787

AUTHOR

Bachar Blal

showing 2 related works from this author

Biotization of microplants for improved performance

2002

International audience

[SDV] Life Sciences [q-bio][SDV]Life Sciences [q-bio]FRAMBOISIERComputingMilieux_MISCELLANEOUSARTICHAUD
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SCAR-based real time PCR to identify a biocontrol strain (T1) of Trichoderma atroviride and study its population dynamics in soils.

2006

Strains of Trichoderma spp. are known for their antagonistic properties against plant pathogens, some are already on the market, others are under development. In order to launch a strain on the market its perfect identification at the species and strain levels is needed. The aim of this study is to (i) design a SCAR marker for specific identification of strain T1 of Trichoderma atroviride and (ii) monitor population dynamics of this strain in soil by real time PCR. A primer pair targeting a 141-bp fragment enabled specific detection of this strain without cross detection of autochthonous populations of Trichoderma in several field soils. In two soils, population dynamics assessed by real ti…

Microbiology (medical)PopulationBiologycomplex mixturesMicrobiologyPolymerase Chain Reactionlaw.inventionlawREAL-TIME PCReducationDNA FungalMolecular BiologyPolymerase chain reactionSoil MicrobiologyTrichodermaeducation.field_of_studyStrain (chemistry)business.industryFungal geneticsfood and beveragesFungi imperfectiSequence Analysis DNAbiology.organism_classificationDNA FingerprintingSOILSBiotechnologyRandom Amplified Polymorphic DNA TechniquePOPULATION DYNAMICSSCAR[SDV.MP]Life Sciences [q-bio]/Microbiology and ParasitologyTrichodermabusinessSoil microbiologySpecific identificationJournal of microbiological methods
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