0000000000699374
AUTHOR
Jürgen E. Scherberich
Novel Monoclonal Antibody 138H11 Against Human γ-Glutamyl-Transferase: Classification, Histogenesis and Immunoscintigraphy of Renal Tumors
Monoclonal antibody (mAb) 138H11 was produced against human gamma-glutamyl transferase as a molecular probe for renal cancer. Immunohistochemical analyses of mAb 138H11 revealed its reaction with the brush border of normal kidney cortex as well as with clear cell and chromophilic renal carcinomas. In contrast, renal oncocytomas, chromophobic carcinomas, and Duct-Bellini carcinomas were negative for this antibody. These results support a new approach for the histogenesis and classification of renal tumors. Immunoscintigraphy of tumor bearing human kidneys, perfused with 99Tcm-labeled mAb 138H11 after nephrectomy, showed enrichment of 138H11 in three of three clear cell carcinomas that was up…
Differential diagnosis of histogenetically distinct human epithelial renal tumours with a monoclonal antibody against gamma-glutamyltransferase.
The localization of membrane-bound gamma-glutamyltransferase with monoclonal antibody (mAb) 138H11 proved to be of value for differential diagnosis of renal cancer, since it correlated with the histogenetic profile of human epithelial renal tumors. Immunoreactive gamma-glutamyltransferase was located in the proximal tubule in all normal human kidneys (15/15) examined thus far by both ultrastructural and immunohistochemical techniques. From 68 epithelial renal cancers tested 31/31 clear-cell carcinomas and 15/16 chromophilic carcinomas expressed the target epitope of mAb 138H11. In contrast, 0/11 oncytomas, 0/9 chromophobic carcinomas, and 0/1 Duct-Bellini carcinoma were immunoreactive. Thes…