0000000000707526
AUTHOR
J. Bode
The Influence of (2-Chloroethyl)trimethylammoniumchloride (CCC) on Growth and Photosynthetic Metabolism of Young Wheat Plants (Triticum aestivum L.)
Summary The influence of (2-chloroethyl)trimethylammoniumchloride on growth and cell metabolism of Triticum aestivum var. Kolibri was investigated. CCC was added to the nutrient solution on the 11th day of development with a final concentration of 10 −2 mol·1 −1 . It immediately stopped root growth and showed a strong inhibitory influence on the growth of the leaves. Smaller and fewer cells accounted for the reduction in final leaf size of about 40 %. The content of soluble proteins and soluble reducing sugars were significantly increased in the treated leaves. CCC also exhibited a stimulating effect on the synthesis of RubPc-ase protein and the in vitro activity of this enzyme. In contrast…
The accumulation of proline in needles of damaged spruces
Im Rahmen einer vergleichenden physiologischen und cytologischen Charakterisierung geschadigter und schadsymptomfreier Fichten am naturlichen Standort wurden im Spatsommer und Herbst 1984 erste Untersuchungen zum Wasserhaushalt der Baume durchgefuhrt. Messungen des Gesamtwasserpotentials, des osmotischen Potentials, des Gehaltes an verschiedenen Kohlenhydraten und an der freien Aminosaure Prolin deuten auf Veranderungen im Wasserhaushalt geschadigter Fichten. Besonders signifikante Ergebnisse waren beim Prolin zu verzeichnen. Wahrend bei den symptomfreien Fichten der Prolingehalt der Nadeln ungeachtet des Standortes und der Witterungsverhaltnisse konstant niedrig lag, trat bei Schadigung ei…
Ribulose-1,5-bisphosphate Carboxylase-Oxygenase: New Aspects Respective the pH-Dependance of the Carboxylation Reaction
The investigation was directed towards the effects of reaction conditions, substrates and pH on the carboxylation reaction of ribulose-1 ,5-bisphosphate carboxylase-oxygenase in the crude enzyme extracts from several plants. Optimal substrate concentrations (HCO3 - and RubP) were determined. The highest carboxylase activity was attained with Tris/HCl buffer. The pH activity profile was quite sharp with an optimum at pH 7.8. Purified and crystallized carboxylase yielded a broad optimum curve under the same reaction conditions