Micro - Architectural Data Extraction for Electrospun Poly (Ester Urethane) Urea Scaffolds for Biomechanical Modeling.

Problem: Soft tissue engineered applications have raised the need for accurate descriptions of tissue microstructure and their contributions to global mechanical behavior [1]. Accurate material image analysis is crucial to model engineered tissue biomechanics. The present study proposes a novel method to automatically collect micro-architectural data from electron micrographs (SEM) of electrospun poly (ester urethane) urea (PEUU). Methods: Sets of contiguous SEM images for electrospun PEUU scaffolds made using three mandrel collection tangential velocities (1.5, 4.5, 9.0 m/s) were analyzed. A combination of thresholding and morphological procedures enabled overlaps of fibers to be detected.…

A novel approach to fully characterize fiber network morphology of planar fibrous tissues and scaffolds

Understanding how scaffold structure influences cell morphology, metabolism, phenotypic expression, and predicting mechanical behaviors have increasingly become crucial goals in the development of engineered tissue scaffolds. A novel image-based analysis algorithm that provides an automatic tool to characterize engineered tissue fiber network topology is presented. Micro architectural descriptors that unambiguously define the fiber network topology were detected, which include fiber orientation distribution, connectivity, intersection spatial density, and diameter. Algorithm performance was tested using actual sample scanning electron microscopy (SEM) images of (1) electrospun poly(ester ur…

Micro Scale Based Mechanical Models for Electrospun Poly (Ester Urethane) Urea Scaffolds.

Micro scale based mechanical models can provide a tool to guide tissue engineering scaffold design and to investigate on how the cellular mechanical and metabolic response are related to local micro-structural deformations. The present study proposes a novel approach to automatically collect micro-architectural data from SEM images of electrospun poly (ester urethane) urea (PEUU) and to recreate statistically equivalent scaffold mechanical models. Sets of contiguous SEM images for each of the three mandrel velocities (1.5, 4.5, 9.0 m/s) were analyzed. A combination of thresholding and morphological procedures enabled fibers overlaps to be detected. The algorithm precision was tested on regu…

Fabrication Techniques for Electrospun Polyurethane Scaffolds that Generate Valve Leaflet Mechanical Properties.

Micro-Architecture Based Structural Model for Elastomeric Electrospun Scaffolds.

Effects of Rastering Velocity on Electrospun Polyeurthane Structure and Mechanical Properties

Analysis of Electrospun Scaffolds Micro - Architectural Data.

Micro-Meso Scale Models of Electrosun Poly (Ester Urethane) Urea Scaffolds.

A Structural Deterministic Model for Electrospun Scaffolds.