0000000000708567
AUTHOR
R. Defez
Introduction of a novel pathway for IAA biosynthesis to rhizobia alters vech root nodule developmt
We introduced into Rhizobium leguminosarum bv. viciae LPR1105 a new pathway for the biosynthesis of the auxin, indole-3-acetic acid (IAA), under the control of a stationary phase-activated promoter active both in free-liv- ing bacteria and bacteroids. The newly introduced genes are the iaaM gene from Pseudomonas savastanoi and the tms2 gene from Agrobacterium tumefaciens. Free-living bacteria harbouring the promoter-iaaMtms2 construct release into the growth medium 14-fold more IAA than the wild-type parental strain. This IAA overproducing R. l. viciae, the RD20 strain, elicits the development of vetch root nodules containing up to 60-fold more IAA than nodules infected by the wild-type str…
Preliminary Results on Biotization of Encapsulated Vitro-Derived Propagules of Carrizo Citrange [Citrus sinensis (L.) Osb. × Poncirus trifoliata (L.) Raf].
The encapsulation technology represents a new tool to integrate micropropagation into the nursery activity. It allows combining the advantages of zygotic or gamic seeds with those of micropropagation. The synthetic or artificial seeds have been defined as “artificially encapsulated somatic embryos, shoots or other tissues which can be used for sowing under in vitro or ex vitro conditions”. This will be a powerful propagation tool in the nurseryman hands, if the levels of the synthetic seeds conversion can be increased also in the nurseries, without the asepsis of in vitro laboratories and with the presence of many parasitic microorganisms, like bacteria and fungi, responsible for contaminat…