0000000000711549
AUTHOR
Bao-shan Zhao
Enhanced extraction of astragalosides from Radix Astragali by negative pressure cavitation-accelerated enzyme pretreatment.
The optimal conditions for extraction of astragalosides III and IV (AGs III and IV) in Radix Astragali by negative pressure cavitation-accelerated enzyme pretreatment were studied on the basis of a Box-Behnken design and response surface methodology. Experimental results showed that negative pressure, amount of enzyme and incubation temperature were the main factors governing the enzyme pretreatment of Radix Astragali. The optimum parameters were obtained as follows: negative pressure -0.08 Mpa, amount of enzyme 1.48% (w/w of materials) and incubation temperature 45 degrees C. Under the optimal conditions, the maximal extraction yields of AGs III and IV were 0.103 and 0.325 mg/g, which were…
Enhanced extraction of isoflavonoids from Radix Astragali by incubation pretreatment combined with negative pressure cavitation and its antioxidant activity
Abstract Incubation pretreatment with the function of endogenous enzyme combined with negative-pressure cavitation extraction (IP-NPCE) was established to extract calycosin and formononetin from Radix Astragali. Significant factors involved in the process were selected using Plackett–Burman factorial design (PBD) and then were optimized by central composite design (CCD). The optimum experimental conditions were incubation at 35 °C, 60 min, pH 4.0 and extraction at negative pressure − 0.080 MPa, ethanol concentration 60%, 60 mesh, 30 min, solid-to-liquid ratio 1:25 and two cycles. Under the optimal conditions, the extraction yields of calycosin and formononetin were 0.650 ± 0.015 and 0.307 ±…
Preparation and antioxidant activity of Radix Astragali residues extracts rich in calycosin and formononetin
Abstract Radix Astragali is considered an excellent source of Traditional Chinese Medicine and a kind of famous restorative food. To make full usage of Radix Astragali, calycosin and formononetin were enriched through various macroporous resins from its residues, HPD500 resin, compared with other resins, offered the best effectiveness for the enrichment and separation of compounds. 35 °C was selected for its highest adoption capacity during static adsorption process, which can be obtained from adsorption isotherms. And then dynamic adsorption and desorption tests were carried out to optimize the operating parameters. After one run treatment with HPD500 resin, the contents of calycosin and f…