6533b82dfe1ef96bd1290b00
RESEARCH PRODUCT
Enhanced extraction of astragalosides from Radix Astragali by negative pressure cavitation-accelerated enzyme pretreatment.
Yuangang ZuWei LiuYu-jie FuThomas EfferthCai-yun ChenBao-shan ZhaoMing-ming Yansubject
Environmental EngineeringScanning electron microscopeBioengineeringPressureRadixResponse surface methodologyWaste Management and Disposalchemistry.chemical_classificationAnalysis of VarianceChromatographyRenewable Energy Sustainability and the EnvironmentChemistryExtraction (chemistry)Reproducibility of ResultsGeneral MedicineAstragalus PlantAstragalus propinquusSaponinsEnzymesEnzymeIncubation temperatureCavitationMicroscopy Electron ScanningRegression AnalysisBiotechnologyDrugs Chinese Herbaldescription
The optimal conditions for extraction of astragalosides III and IV (AGs III and IV) in Radix Astragali by negative pressure cavitation-accelerated enzyme pretreatment were studied on the basis of a Box-Behnken design and response surface methodology. Experimental results showed that negative pressure, amount of enzyme and incubation temperature were the main factors governing the enzyme pretreatment of Radix Astragali. The optimum parameters were obtained as follows: negative pressure -0.08 Mpa, amount of enzyme 1.48% (w/w of materials) and incubation temperature 45 degrees C. Under the optimal conditions, the maximal extraction yields of AGs III and IV were 0.103 and 0.325 mg/g, which were 41.67% and 65.31% increased as compared to those without enzyme pretreatment, respectively. The effect of negative pressure cavitation and enzyme pretreatment on the structural changes of plant cells was observed by scanning electron microscopy. In conclusion, negative pressure cavitation-accelerated enzyme pretreatment was proved to be environment-friendly and economical, and could be used in secondary metabolites production.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2010-02-25 | Bioresource technology |