0000000000713294

AUTHOR

Virginie Serpaggi

showing 5 related works from this author

Partial vinylphenol reductase purification and characterization from Brettanomyces bruxellensis

2008

International audience; Brettanomyces is the major microbial cause for wine spoilage worldwide and causes significant economic losses. The reasons are the production of ethylphenols that lead to an unpleasant taint described as 'phenolic odour'. Despite its economic importance, Brettanomyces has remained poorly studied at the metabolic level. The origin of the ethylphenol results from the conversion of vinylphenols in ethylphenol by Brettanomyces hydroxycinnamate decarboxylase. However, no information is available on the vinylphenol reductase responsible for the conversion of vinylphenols in ethylphenols. In this study, a vinylphenol reductase was partially purified from Brettanomyces bruxe…

Chromatography GasBrettanomycesMolecular Sequence DataVINYLPHENOL REDUCTASEBrettanomyces bruxellensisWineReductaseMicrobiology[ CHIM ] Chemical SciencesFungal Proteins03 medical and health sciencesHydrolysisOpen Reading FramesPhenolsOxidoreductaseGenetics[CHIM]Chemical SciencesAmino Acid SequenceMolecular Biology030304 developmental biologychemistry.chemical_classificationWineVOLATILE PHENOL0303 health sciencesbiology030306 microbiologyChemistryGuaiacolTemperatureBRETTANOMYCESHydrogen-Ion Concentrationbiology.organism_classificationNADAmino acidMolecular WeightKineticsEnzymeBiochemistryDETERIORATION MICROBIENNESaccharomycetalesBRUTTANOMYCES BRUXELLENSISFood MicrobiologyElectrophoresis Polyacrylamide GelOxidoreductases
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Characterization of the "viable but nonculturable" (VBNC) state in the wine spoilage yeast Brettanomyces.

2012

Although the viable but not culturable (VBNC) state has been studied in detail in bacteria, it has been suggested that maintenance of viability with loss of culturability also exists in eukaryotic cells, such as in the wine spoilage yeast Brettanomyces. To provide conclusive evidence for the existence of a VBNC state in this yeast, we investigated its capacity to become viable and nonculturable after sulfite stress, and its ability to recover culturability after stressor removal. Sulfite addition induced loss of culturability but maintenance of viability. Increasing the medium pH to decrease the concentration of toxic SO(2) allowed yeast cells to become culturable again, thus demonstrating …

volatile phenol[SDV.SA]Life Sciences [q-bio]/Agricultural sciencessynthetic wineBrettanomycesFood spoilageviable but nonculturableBrettanomyces bruxellensisBrettanomycesWineyeastMicrobiologyViable but nonculturableMicrobiology03 medical and health sciencesSulfur DioxideFood scienceproteomic030304 developmental biologyWine0303 health sciencesbiology030306 microbiologyflow cytometrybiology.organism_classificationYeastCulture MediaYeast in winemakingBrettanomyces bruxellensisBacteriaFood ScienceFood microbiology
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Specific Identification and Quantification of the Spoilage Microorganism Brettanomyces in Wine by Flow Cytometry: A Useful Tool for Winemakers

2010

International audience; no abstract

HistologyBrettanomycesMicroorganismFood spoilageBrettanomycesWine[ CHIM ] Chemical SciencesPathology and Forensic MedicineFlow cytometry03 medical and health sciencesmedicine[CHIM]Chemical SciencesFood scienceIn Situ HybridizationComputingMilieux_MISCELLANEOUS030304 developmental biologyWine0303 health sciencesmedicine.diagnostic_testbiology030306 microbiologyChemistryCell BiologyFlow Cytometrybiology.organism_classificationFermentationFermentationSpecific identification
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Diversité des Brettanomyces et de leur résistance au SO2. Les nouvelles avancées vers une meilleure gestion du SO2 en vinification.

2019

International audience; Des recherches ont été menées par le Groupe National « Lutte contre Brettanomyces » et plus particulièrement sur la relation SO2 et Brettanomyces bruxellensis afin d’approfondir les connaissances sur le comportement de la levure et d’apporter des données essentielles à une bonne gestion du risque.Une grande diversité de la levure Brettanomyces a été mise en évidence (identification de différents groupes génétiques) ainsi que des comportements différents vis-à-vis du SO2 : sensibles, tolérants ou résistant. Grâce à la mise au point d’un outil prédictif (TYP \ Brett), les professionnels pourront connaître le groupe génétique pour mieux intervenir.Ces travaux ont égalem…

Résistance au SO2LevuresSulfite[SDV.BV]Life Sciences [q-bio]/Vegetal BiologyBrettanomycesGestion du SO2[SDV.BV] Life Sciences [q-bio]/Vegetal BiologyVinification4-éthylphénol
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Quantification des Brettanomyces par qPCR

2017

International audience; Brettanomyces bruxellensis est une levure d’altération du vin avec de faibles besoins nutritionnels, résistante à l’éthanol et aux faibles pH, lui permettant une implantation en vin durant ou après la fermentation alcoolique (Conterno et al., 2006). B. bruxellensis est capable de produire des phénols volatils (éthyl-4-phénol, éthyl-4-gaïacol et éthyl-4-catéchol) (Oelofse et al., 2008). Ces molécules volatiles odorantes amènent un caractère phénolé et animal au vin connu sous le nom de « caractère Brett ». D’autres molécules (2-acétyltétrahydropyridine et 2-éthyltétrahydropyridine) produites par B. bruxellensis sont également responsables d’une déviation organoleptiqu…

Laboratoirekits commerciauxPCR quantitativeVin rouge[ SDV.IDA ] Life Sciences [q-bio]/Food engineeringExtraction d'ADN[SDV.IDA] Life Sciences [q-bio]/Food engineeringBordeauxCôtes-du-Rhône[SDV.IDA]Life Sciences [q-bio]/Food engineeringBrettanomycesbruxellensisBourgogneFiabilitéRépétabilitéReproductibilité
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