0000000000727080

AUTHOR

Josep Romero

showing 3 related works from this author

Fertilization after intracytoplasmic sperm injection with cryopreserved testicular spermatozoa.

1996

Objective To assess the possibility of cryopreserving testicular tissue extracted sperm for intracytoplasmic sperm injection (ICSI). Design A report of two cases. Our study was approved by the Ethical Committee at the Instituto Valenciano de Infertilidad. Setting In vitro fertilization program at the Instituto Valenciano de Infertilidad. Patients Two azoospermic patients with severe spermatogenic failure but with focal spermatogenesis on testicular biopsies. In both cases, a first ICSI attempt with fresh testicular biopsy extracted sperm was unsuccessful. Interventions Cryopreservation of testicular spermatozoa in 100-µL "pills." Intracytoplasmic sperm injection with thawed testicular sperm…

AdultMaleendocrine systemmedicine.medical_specialtyendocrine system diseasesMicroinjectionsmedicine.medical_treatmentBiopsyFertilization in VitroBiologyurologic and male genital diseasesIntracytoplasmic sperm injectionCryopreservationAndrologyTestismedicineHumansreproductive and urinary physiologyAzoospermiaGynecologyCryopreservationIn vitro fertilisationurogenital systemObstetrics and GynecologyOligospermiamedicine.diseaseSpermSpermatozoaTesticular sperm extractionReproductive MedicineFemaleSpermatogenesisEmbryo qualityFertility and sterility
researchProduct

Storage of human oocytes in the vapor phase of nitrogen

2009

Objective To evaluate the effectiveness of long-term vapor-phase nitrogen storage of vitrified human oocytes as a strategy for preventing the risk of cross-contamination due to direct contact with the liquid nitrogen (LN). Design Prospective randomized study. Setting Private infertility center, IVI, Valencia. Patient(s) Oocyte donors (n = 44) and recipients (n = 46). Intervention(s) Vitrification by the Cryotop method. Storage of vitrified oocytes in a vapor-phase nitrogen storage freezer and a traditional LN storage tank. Donation of the surviving oocytes and evaluation of fertilization, embryo development, and clinical results. Main Outcome Measure(s) Survival, fertilization, and cleavage…

Adultmedicine.medical_specialtyPregnancy RateCell SurvivalNitrogenFertilization in VitroAndrologyHuman fertilizationPregnancymedicineHumansVitrificationProspective StudiesBlastocystCryopreservationGynecologyChemistryTemperatureObstetrics and GynecologyMiddle AgedLiquid nitrogenEmbryo TransferOocyteEmbryo transferPregnancy rateTreatment Outcomemedicine.anatomical_structureReproductive MedicineOocytesFemaleInfertility FemaleCell DivisionEmbryo qualityFertility and Sterility
researchProduct

TESTICULAR SPERM EXTRACTION AND INTRACYTOPLASMIC SPERM INJECTION

1998

We evaluate the efficacy of testicular sperm extraction and results of intracytoplasmic sperm injection in cases of nonobstructive azoospermia. In addition, we define predictive parameters for successful testicular sperm extraction in these patients.A total of 154 patients with nonobstructive azoospermia underwent multiple testicular biopsies to obtain testicular spermatozoa and for histopathological diagnosis. Results of testicular sperm extraction were related to suspected etiology of azoospermia, patient age, maximal testicular volume, serum follicle-stimulating hormone and histopathology. When testicular sperm extraction was successful, intracytoplasmic sperm injection was performed.Spe…

AdultMaleCytoplasmendocrine systemendocrine system diseasesmedia_common.quotation_subjectmedicine.medical_treatmentUrologySemenFertilityFertilization in VitroSuctionTesticleurologic and male genital diseasesIntracytoplasmic sperm injectionInjectionsAndrologyHuman fertilizationPregnancyTestisHumansMedicinereproductive and urinary physiologyOvummedia_commonAzoospermiaurogenital systembusiness.industryOligospermiamedicine.diseaseSpermatozoaTesticular sperm extractionmedicine.anatomical_structureOligospermiaFemalebusinessThe Journal of Urology
researchProduct