0000000000732228
AUTHOR
E. Lesniewska
Les nanotechnologies au service du développement du vin
National audience; Vous attendez une nouvelle révolution ? Vous souhaitez élaborer des méthodes d’investigation sur les cellules de vigne, les levures, les bactéries, des procédures d’identification de pesticides, des biocapteurs qui pourront détecter des molécules spécifiques comme le resvératrol, des nanorobots qui pourront pénétrer à l'intérieur des bouteilles pour évaluer la qualité d’un vin... Les nanotechnologies vous permettent de refaire le monde atome par atome, molécule par molécule, d’assembler des nano-objets en vue d’applications particulières : modification des propriétés du conditionnement pour garder l’oxygène à distance, le nettoyage ionique... Les nanotechnologies peuvent …
Cell imaging connected with protein mapping to investigate glyphosate induced toxicity on human keratinocytes
During the skin ageing which is accelerated by environmental factors, the generated reactive oxygen species make unstable the "proliferation/differentiation/apoptosis" coupling of epidermal cells. A biochemical study, previously realized on human keratinocyte cultures treated by a pesticide, glyphosate, allowed the validation of an in vitro model of skin ageing. In this work, we propose to study the loss of HaCaT cell integrity appearing after this chemical induced-oxidative stress. An original approach, combining a micro- to nanoscale cell characterization and analysis of fluctuating protein expression, is proposed: a) the exploration of cell growth and morphology through fluorescence and …
Concept of dynamic DNA network dedicated to DNA-Protein interactions studies
The approach lies on the development of an original DNA biosensor. This self-assembled DNA biochip is constituted of a tethered lipid membrane presenting « protein/DNA » complexes. This unique assembly, using a highly flexible bifunctional molecular spacer and a fluidic matrix, allows the control of oligonucleotides surface density and their mobility thus favouring hybridization of the DNA probes in liquid environment and under weak constraints. Recent Surface Plasmon Resonance experiments lead to building of protein/DNA dimers presenting two special DNA sequences recognized by regulation proteins. We demonstrated the capacity and relevance of this biosensor in the case of an estrogen recep…