A New Culture Technique: An Approach to the in-Vitro Reconstitution of Endocrine Organs

A new cell culture technique called three-dimensional-rotation culture (3dR-culture) has been developed. Its main characteristics are: (i) cultivation under constant rotation, and (ii) the use of a three-dimensional material that allows the cells to adhere and to grow in a three-dimensional arrangement. Two glassfibre materials and one gelatine-sponge proved suitable for the use in cell culture. The new technique has the advantage to yield in large numbers cells bound on a stable matrix that can easily be handled, e.g. for implantation.