0000000000739535
AUTHOR
Xavier Foissac
Génomique du phytoplasme de la Flavescence Dorée
National audience
Epidémiologie moléculaire et diversité des phytoplasmes du groupe 16SrV présents dans les compartiments viticoles et sauvages en Europe
National audience
Comparison of prevalence, geographical distribution and biological properties of two flavescence dorée phytoplasma strains
National audience
Impact of vector dispersal and host-plant fidelity on the dissemination of an emerging plant pathogen
International audience; Dissemination of vector-transmitted pathogens depend on the survival and dispersal of the vector and the vector's ability to transmit the pathogen, while the host range of vector and pathogen determine the breath of transmission possibilities. In this study, we address how the interaction between dispersal and plant fidelities of a pathogen (stolbur phytoplasma tuf-a) and its vector (Hyalesthes obsoletus: Cixiidae) affect the emergence of the pathogen. Using genetic markers, we analysed the geographic origin and range expansion of both organisms in Western Europe and, specifically, whether the pathogen's dissemination in the northern range is caused by resident vecto…
Sequence typing of map, secY and degV genes confirm the genetic relatedness of three distinct groups of flavescence dorée phytoplasmas with phytoplasmas of the 16SrV group infecting grapevine and alder in Europe
International audience
Multilocus sequence typing confirms the close genetic inter-relatedness between three distinct flavescence doree phytoplasma strain clusters and group 16SrV phytoplasmas infecting grapevine and alder in Europe
ABSTRACT Vineyards of southern France and northern Italy are affected by the flavescence dorée (FD) phytoplasma, a quarantine pathogen transmitted by the leafhopper of Nearctic origin Scaphoideus titanus . To better trace propagation of FD strains and identify possible passage between the vineyard and wild plant compartments, molecular typing of phytoplasma strains was applied. The sequences of the two genetic loci map and uvrB - degV , along with the sequence of the secY gene, were determined among a collection of FD and FD-related phytoplasmas infecting grapevine, alder, elm, blackberry, and Spanish broom in Europe. Sequence comparisons and phylogenetic analyses consistently indicated the…
A chromosome map of the Flavescence dorée phytoplasma
International audience; The Flavescence dorée phytoplasma (FD-P), a non-cultivable, plant-pathogenic bacterium of the class Mollicutes, is the causal agent of a quarantine disease affecting vineyards of southern Europe, mainly in southern France and northern Italy. To investigate FD-P diversity and phytoplasma genetic determinants governing the FD-P life cycle, a genome project has been initiated. A physical map of the chromosome of FD-P strain FD92, purified from infected broad beans, was constructed by performing restriction digests of the chromosome and resolving the fragments by PFGE. Single and double digestions of the chromosome with the enzymes SalI, BssHII, MluI and EagI were perfor…
Progress towards a chromosome map of the “flavescence dorée” phytoplasma
International audience
Map gene as a new non ribosomal marker to study the molecular variability of grapevine yellows and related phytoplasmas from group 16SRV in Europe
International audience