0000000000740808
AUTHOR
Inese Kokina
Target Transportation of Auxin on Mesoporous Au/SiO2 Nanoparticles as a Method for Somaclonal Variation Increasing in Flax (L. usitatissimum L.)
Development of methods for direct delivery of different bioactive substances into the cell is a promising and intensively approached area of research. It has become a subject of serious research for multidisciplinary team of scientists working in such areas as physics, biology, and biotechnology. Plant calluses were grown on medium supplemented with different nanoparticles to be used as a model for biotechnological research. Gold nanoparticles with mesoporous silica coating were used as hormone carriers, since they possess many of critical properties required for cellular transportation instrument. Some of those properties are great biocompatibility and controlled release of carried molecul…
The Impact of CdS Nanoparticles on Ploidy and DNA Damage of Rucola (Eruca sativa Mill.) Plants
The genotoxic effect of cadmium sulfide nanoparticles (CdS NPs) of different sizes in rucola (Eruca sativaMill.) plants was assessed. It was confirmed that nanoparticles < 5 nm in size were more toxic than larger particles at an identical mass concentration. Significant differences in cell ploidy, as well as in the mitotic index, were detected between control and treated samples. Differences in the DNA banding pattern between control samples and samples after treatment with cadmium sulfide nanoparticles were significant and detected at different places as the appearance or elimination of DNA fragments. Fluorescence images showed that cadmium sulfide nanoparticles smaller than 5 nm in siz…
The peculiarities of genetic structure of the Blumeria graminis f. sp. hordei population in Lithuania
In 2010, samples of Blumeria graminis f. sp. hordei both in sporulation and cleistothecia were collected in Lithuania from spring barley variety ‘Dina’. Eighty monopustule isolates were tested on differentials with well-known resistance genes. Frequencies of virulence genes, virulence complexity and pathotypes were detected. Virulence frequencies showed a wide range from 0 to 90%. The clear tendency of virulence increasing for Vla in cleistothecia was observed during the growing season of the pathogen. No virulences were found against resistances, which were present in the line SI1, as well as no matching virulence was found for the resistance gene mlo in Lithuania in 2010. Wide diversity o…