0000000000741418
AUTHOR
Nathalie Hue
Additional file 3: of Transcriptome analysis revealed that a quorum sensing system regulates the transfer of the pAt megaplasmid in Agrobacterium tumefaciens
Mutagenesis of the quorum sensing determinants of pAtP4. All mutations were generated by the insertion of the same Gm resistance cassette that originated from p34S-Gm [39] either at the XbaI site of the cinI gene or at a reconstituted SphI site generated by the fusion of parts of cinR and cinX determinants in a synthetic ORF. (PDF 46Â kb)
Additional file 4: of Transcriptome analysis revealed that a quorum sensing system regulates the transfer of the pAt megaplasmid in Agrobacterium tumefaciens
The 32 differentially expressed genes identified by RNA-seq. Only changes above 3 fold with a p-value lower than 5 10 â 2 were considered significant. All differentially expressed genes were upregulated in P4 when compared to P4cinI. (PDF 285Â kb)
Additional file 2: of Transcriptome analysis revealed that a quorum sensing system regulates the transfer of the pAt megaplasmid in Agrobacterium tumefaciens
UPLC MS-MS solvent composition. The table provides the composition of the solvent mixture used in the UPLC MS-MS analyses of the concentrated extract of the culture supernatant of strain P4. (DOCX 14Â kb)
Additional file 1: of Transcriptome analysis revealed that a quorum sensing system regulates the transfer of the pAt megaplasmid in Agrobacterium tumefaciens
Map of the genetic determinants involved in Ti plasmid conjugation in Agrobacterium strain C58. The functions of operons or genetic regions are as follows: acc, agrocinopine catabolism; arc, agrocinopine regulation of conjugation; noc, nopaline catabolism; tra, conjugative transfer region a; trb conjugative transfer region b; vir, virulence. Both the acc and arc operons are regulated by the repressor AccR. In the presence of agrocinopines A and B or in an accR mutant, the acc and arc operons are expressed. The expression of the arc operon permits the production of the QS regulator TraR. The production of the QS signal 3O,C8-HSL by the synthase TraI leads to an accumulation of the signal unt…
Transcriptome analysis revealed that a quorum sensing system regulates the transfer of the pAt megaplasmid in Agrobacterium tumefaciens.
Background Agrobacterium tumefaciens strain P4 is atypical, as the strain is not pathogenic and produces a for this species unusual quorum sensing signal, identified as N-(3-hydroxy-octanoyl)-homoserine lactone (3OH,C8-HSL). Results By sequence analysis and cloning, a functional luxI-like gene, named cinI, has been identified on the At plasmid of A. tumefaciens strain P4. Insertion mutagenesis in the cinI gene and transcriptome analyses permitted the identification of 32 cinI-regulated genes in this strain, most of them encoding proteins responsible for the conjugative transfer of pAtP4. Among these genes were the avhB genes that encode a type 4 secretion system (T4SS) involved in the forma…
Additional file 5: of Transcriptome analysis revealed that a quorum sensing system regulates the transfer of the pAt megaplasmid in Agrobacterium tumefaciens
Differentially expressed genes. Xlsx. Four repeats are show for the mutant strain P4cinI (col. B to E) with repetition numbers (e.g. rep1_cinI) and three repeats for the wild type strain P4 (col. F to H), also with repetition numbers (e.g. rep1_P4). Average values are given for the mutant strain P4cinI (col. I) and the wild type strain P4 (col. J). Fold change and Log2 fold change are also shown (col. K and L) along with the raw p values and adjusted p values (col. M and N). (XLSX 15Â kb)