0000000000744952

AUTHOR

Siân Astley

showing 2 related works from this author

Bioavailability and metabolism.

2002

Food intakefood intakephenol derivativeClinical BiochemistryGlucosinolatesreviewantioxidant activityBiological AvailabilityPhysiological SciencesAscorbic AcidBiologydigestionalpha tocopherolBiochemistryIntestinal absorptionmedical researchAntioxidantsSeleniumPhenolsLife ScienceAnimalsHumansVitamin ETissue DistributionTissue distributionhumanglutathioneMolecular BiologynonhumanWageningen Food Safety ResearchfoodGeneral MedicineMetabolismAscorbic acidCarotenoidsGlutathionecarotenoidBioavailabilityBiochemistryIntestinal AbsorptionHealthFoodOrgan SpecificityPhenol derivativeMolecular MedicinebioavailabilityabsorptionmetabolismBiological availabilityMolecular aspects of medicine
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Inter-laboratory validation of procedures for measuring 8-oxo-7,8-dihydroguanine/8-oxo-7,8-dihydro-2’-deoxyguanosine in DNA.

2002

The aim of ESCODD, a European Commission funded Concerted Action, is to improve the precision and accuracy of methods for measuring 8-oxo-7,8-dihydroguanine (8-oxoGua) or the nucleoside (8-oxodG). On two occasions, participating laboratories received samples of different concentrations of 8-oxodG for analysis. About half the results returned (for 8-oxodG) were within 20% of the median values. Coefficients of variation (for three identical samples) were commonly around 10%. A sample of calf thymus DNA was sent, dry, to all laboratories. Analysis of 8-oxoGua/8-oxodG in this sample was a test of hydrolysis methods. Almost half the reported results were within 20% of the median value, and half …

GuanineAnalytical chemistryTest sensitivityThymus GlandSensitivity and SpecificityBiochemistryGas Chromatography-Mass SpectrometryMass SpectrometryOxidative dna damagechemistry.chemical_compound8 oxo 7 8 dihydroguanineAnimalsHumansEuropean commissionInter-laboratoryChromatography High Pressure LiquidChromatographyChemistry8 oxo 7 8 dihydro 2 deoxyguanosineDNAGeneral MedicineCattleBiomarkersDNAChromatography LiquidDNA Damage
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