0000000000744969

AUTHOR

William J. Britt

showing 2 related works from this author

Ablation of the Regulatory IE1 Protein of Murine Cytomegalovirus Alters In Vivo Pro-inflammatory TNF-alpha Production during Acute Infection

2012

Little is known about the role of viral genes in modulating host cytokine responses. Here we report a new functional role of the viral encoded IE1 protein of the murine cytomegalovirus in sculpting the inflammatory response in an acute infection. In time course experiments of infected primary macrophages (MΦs) measuring cytokine production levels, genetic ablation of the immediate-early 1 (ie1) gene results in a significant increase in TNFα production. Intracellular staining for cytokine production and viral early gene expression shows that TNFα production is highly associated with the productively infected MΦ population of cells. The ie1- dependent phenotype of enhanced MΦ TNFα production …

MaleCytomegalovirus InfectionMuromegalovirusViral Diseasesmedicine.medical_treatmentvirusesTNF TNF-alpha murine cytomegalovirus MCMV IEVirus ReplicationMice0302 clinical medicineGene expressionBiology (General)Mice Inbred BALB C0303 health scienceseducation.field_of_studyPhysicsvirus diseasesHerpesviridae InfectionsTransfection3. Good healthGenètica microbianaInterleukin 10PhenotypeInfectious DiseasesCytokineLiverCytokinesMedicineFemaleTumor necrosis factor alphaBIOMEDICINA I ZDRAVSTVO. Temeljne medicinske znanosti.Microbial geneticsSignal TransductionResearch ArticleDNA ReplicationGene Expression Regulation ViralQH301-705.5ImmunologyPopulationBiologyMicrobiologyCell LineImmediate-Early ProteinsViral Proteins03 medical and health sciencesIn vivoVirologyGeneticsmedicineAnimalseducationMolecular Biology030304 developmental biologyTumor Necrosis Factor-alphaMacrophagesBIOMEDICINE AND HEALTHCARE. Basic Medical Sciences.FísicaRC581-607Mice Inbred C57BLViral replicationDNA ViralImmunologyParasitologyImmunologic diseases. Allergy030215 immunology
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Cytomegalovirus vector expressing RAE-1γ induces enhanced anti-tumor capacity of murine CD8+ T cells

2017

Designing of CD8 T cell vaccines which would provide protection against tumors is still considered a great challenge in immunotherapy. Here we show a robust potential of a cytomegalovirus (CMV) vector expressing the NKG2D ligand RAE-1γ as CD8 T cell-based vaccine against malignant tumors. Immunization with the CMV vector expressing RAE-1γ delayed tumor growth or even provided complete protection against tumor challenge in both prophylactic and therapeutic settings. Moreover, a potent tumor control in mice vaccinated with this vector can be further enhanced by blocking the immune checkpoints TIGIT and PD-1. Expression of RAE-1γ by the CMV vector potentiated expansion of KLRG1+ CD8 T cells wi…

0301 basic medicineTumor vaccine [RAE-1γ]medicine.medical_treatmentT cellImmunologyGenetic VectorsProgrammed Cell Death 1 ReceptorMelanoma ExperimentalCytomegalovirusEpitopes T-LymphocyteBiologyCD8-Positive T-LymphocytesCancer VaccinesArticleCMV vectorNKG2DImmunomodulation03 medical and health sciencesMiceImmune systemTIGITKLRG1+ CD8+ T cellsNeoplasmsmedicineImmunology and AllergyCytotoxic T cellAnimalsHumansRAE-1γ : Tumor vaccineLectins C-TypeReceptors ImmunologicαTIGIT ; CMV vector ; KLRG1+ CD8+ T cells ; NKG2D ; RAE-1γ : Tumor vaccineBIOMEDICINE AND HEALTHCARE. Basic Medical Sciences.Membrane ProteinsImmunotherapyNKG2DVirology3. Good healthKiller Cells NaturalDisease Models Animal030104 developmental biologymedicine.anatomical_structureImmunizationAnimals NewbornFemaleαTIGITImmunotherapyBIOMEDICINA I ZDRAVSTVO. Temeljne medicinske znanosti.CD8
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