Analysis of Trastuzumab effects in breast cancer cells in vitro

MULTIOMICS ANALYSIS OF S100 PROTEINS IN BREAST CANCER

The S100 gene family is the largest subfamily of calcium binding proteins of EF-hand type, expressed in tissue and cell-specific manner. S100 proteins act as intracellular regulators and as extracellular signaling. Within cells, S100 have been involved in the regulation of proliferation, differentiation, apoptosis, energy metabolism, inflammation, migration and invasion via interactions with a variety of target proteins. Extracellular S100 proteins act in an autocrine and paracrine manner through the activation of surface receptors that regulate cell proliferation, differentiation, survival and migration. More recently, there is growing interest in the S100 proteins and their relationship w…

NEW PROTEOMIC EVIDENCE ON DECORIN EFFECTS ON BREAST CANCER CELLS

The estabilishment of a dinamic crosstyalk between the malignant cells and several components of the ECM is a crucial step of the tumor progression. The aim of the present study was to improve the knowledge about the effects of ectopic decorin on the breast cancer cells, starting from our previous proteomic studies. The new proteomic evidences strenghteh the anti-oncogenic effects of decorin and hilight the attention on the decreased expression of the majority of the members of three protein classes closely related to the malignant phenotype: the metabolic enzymes, the S100 family and the cell motility proteins

DECORIN EFFECTS ON PROTEOMIC PROFILING OF BREAST CANCER CELLS: AN UPDATED STUDY

The malignant carcinomas are characterized by several capabilities acquired by the neoplastic cells, among which the ability to invade the extracellular matrix (ECM) and to establish a crosstalk with several ECM components. Under this respect, the extracellular microenvironment is an entity extraordinarily rich of information with opposite signals. Our group has long undertaken the study of the effects of ECM molecules on the behavior of cancer cells in vitro. Among the studied molecules, the decorin was found to exert a non-permissive effect on the growth and motility of the transfected tumor cells. The decorin, belongs to the family of small leucine-rich proteoglycans (SLRP) and is involv…

BEHAVIOVRAL CHANGES OF BREAST CANCER CELLS IN VITRO

S100PROTEINS IN BREAST CANCER: MULTIOMICS-BASED ANALYSIS

S100 gene family is the largest subfamily of calcium binding proteins, expressed in tissue and cell-specific manner. Within cells, S100 have been involved in the regulation of proliferation, differentiation, apoptosis, energy metabolism, inflammation, migration and invasion. Extracellular S100 proteins act in an autocrine and paracrine manner and regulate cell proliferation, differentiation, survival and migration. S100 proteins play important roles in the development and progression of tumors due to their multifunctional roles. However, the occurrence, the role and the possible coordination of this group of proteins in breast cancer is still poorly known. We previously describe a large-sca…

PHENOTYPIC PROFILING OF OSTEOTROPIC BREAST CANCER CELLS

One of the preferred locations of metastases from breast cancer is the bone tissue. On the other hand, it should be recalled that mammary tumors with equal clinical diagnosis have a different course, and also different metastatic progression. Therefore, it would be helpful to have appropriate markers of osteotropism to test on the surgical cancer tissues, in order to predict the possible propensity of the breast cancer to generate bone metastases and to adequate the therapeutic plan. We previously reported1,2 on the setting-up of an in vitro model for the study of the osteotropic propensity of breast cancer cells and the influences exerted by the bone microenvironment on the cancer cells ph…

Endothelial cells and normal breast epithelial cells enhance invasion of breast carcinoma cells by CXCR-4-dependent up-regulation of urokinase-type plasminogen activator receptor (uPAR, CD87) expression

Here we show the increase of invasion of three breast cancer cell lines (8701-BC, MDA-MB-231 and SKBR3) upon long-term co-incubation with culture medium of normal microvascular endothelial cells (MVEC) and normal breast epithelial cells (HB2). The enhancement of invasion relied on the interaction of microvascular endothelial cell and normal breast epithelial cell CXCL12 (SDF1) chemokine, whose expression by breast cancer cells was very low, with the cognate CXCR4 receptor of malignant cells, which resulted in over-expression of the urokinase-type plasminogen activator receptor (uPAR) on their surfaces. uPAR over-expression, showed by RT-PCR and Western blotting, was paralleled by increased …

COMPARATIVE PROFILING BY PROTEOMICS AND ZYMOGRAPHIC ACTIVITIES OF TUMORAL AND NON TUMORAL CELL LINES

The extracellular matrix (ECM) underlying epithelial tissues is involved in the maintenance of cell polarity and homeostasis. ECM is a dynamic structure under the regulated remodeling of its components. The major enzymes responsible of matrix degradation are the matrix metalloproteinases (MMPs), a well known family of zinc-dependent endopeptidases. Much attention has been focused on MMP-2 and MMP-9 because of their ability to degrade type IV collagen, a major constituent of basement membranes. A deregulated proteolysis of ECM molecules may cause the alteration of cell polarity and may contribute to the disruption of cell–cell and cell–ECM adhesions, promoting cancer progression. These alter…