0000000000759239
AUTHOR
Peter M. Delaney
Microarchitecture of the Normal Gut Seen with Conventional Histology and Endomicroscopy
For ex vivo histological examination of the gastrointestinal tract, fractions of an organ or small pieces of tissue are needed. Several steps are used in the fixation, staining, and mounting process to ensure production of good-quality histology on glass slides. The most frequently used stain in routine histology is the haematoxylin and eosin (H&E) stain. The most frequently used tissue-staining methods are shown in ⊡ Table 6.1. The final histopathological diagnosis is always based on examination of the whole sample and the structure and architecture of that sample. In cytology, single cells and nuclei are used for making a diagnosis, so staining procedures in cytology are much faster and e…
Simultaneous confocal laser endomicroscopy and chromoendoscopy with topical cresyl violet
Background Confocal laser endomicroscopy (CLE) has been shown to reliably predict histology during ongoing endoscopy. To unmask lesions for CLE, chromoendoscopy has been mandated. Usually fluorescein then serves as a contrast agent for CLE, but it does not allow direct nuclear visualization, must be injected, leads to a transient skin discoloration, and may have allergic side effects. Objective To establish a single topical dye, cresyl violet (CV), for simultaneous chromoendoscopy and in vivo CLE of the lower GI tract. Design Animal preclinical study, prospective clinical trial. Setting Mainz University Clinic (tertiary care center). Patients, Methods, and Interventions To establish the sta…