Purification and Characterization of Acidic Glutathione S-Transferases (EC 2.5.1.18) from Rat Kidney

Glutathione S-transferases (EC 2.5.1.18) (GSTs) are present in almost every mammalian tissue and catalyze the conjugation of glutathione (GSH) with different electrophilic substrates (Habig et al. 1974). In contrast to this deactivation function of GSTs, they also appear to be involved in the activation of certain chemicals, and particularly halogenated compounds, in either the liver or extra-hepatic tissues (even the kidney) to nephrotoxic and potentially nephrocarcin-ogenic products (Anders et al. 1987, Dekant et al. 1986, Elfarra and Anders 1984, Igwe 1986). These previous results confirm the necessity of a deeper insight into the GSH conjugation system of the kidney: firstly, because of…