0000000000761804

AUTHOR

Alba Grifoni

showing 4 related works from this author

Impact of Human Leukocyte Antigen Polymorphisms in Human Immunodeficiency Virus Progression in a Paediatric Cohort Infected with a Mono-phyletic Huma…

2014

Objective: HLA polymorphisms within the peptide binding pocket have been associated with rapid and slowprogression to AIDS, suggesting that the capability to present efficiently HIV-1 epitopes is crucial for the infection control. To minimize the effects of genetic background due to population coming from different geographic area and viral strain variability in the cohort, an analysis of all the polymorphisms associated with the HLA-A, -B and -DR alleles has been performed in a cohort of children with a monophyletic HIV-1 infection (CRF02_AG) during an outbreak in Libya. Methods: High-resolution HLA-typing has been performed in 58 children infected with a monophyletic strain of HIV-1: 26 L…

Settore MED/04 - Patologia GeneraleSevere combined immunodeficiencyeducation.field_of_studybusiness.industryImmunologyPopulationPeptide bindingDermatologyHuman leukocyte antigenOmicsmedicine.diseaseVirologyPathogenesisInfectious DiseasesVirologyImmunologyMedicineAllelebusinesseducationImmunodeficiencyJournal of AIDS & Clinical Research
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A whole blood test to measure SARS-CoV-2-specific response in COVID-19 patients

2021

Objectives To examine whether specific T-cell-responses to SARS-CoV-2 peptides can be detected in COVID-19 using a whole-blood experimental setting, which may be further explored as potential diagnostic tool. Methods We evaluated IFN-γ levels after stimulating whole-blood with spike and remainder-antigens peptides megapools (MP) derived from SARS-CoV-2 sequences; IL-1β, IL-1RA, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12p70, IL-13, IL-15, IL-17A, eotaxin, basic FGF, G-CSF, GM-CSF, IFN-γ, IP-10, MCP-1, MIP-1α, MIP-1β, PDGF, RANTES, TNF-α, VEGF were also evaluated. Results IFN-γ-response to spike and remainder-antigens MPs was significantly increased in 35 COVID-19-patients compare…

Male0301 basic medicinemedicine.medical_treatmentBasic fibroblast growth factorchemistry.chemical_compound0302 clinical medicineT-cell based testsMedicine030212 general & internal medicineIFN-γAntigens ViralMacrophage inflammatory proteinbiologyInterleukinGeneral MedicineMiddle AgedWhole bloodVascular endothelial growth factorInfectious Diseasesmedicine.anatomical_structureSpike Glycoprotein CoronavirusCytokinesOriginal ArticleFemalePlatelet-derived growth factor receptorAdultMicrobiology (medical)Specific response030106 microbiologyCOVID-19 Serological TestingInterferon-gamma03 medical and health sciencesTh2 CellsAntigenHumansImmune responseAgedSARS-CoV-2business.industryGrowth factorMonocyteCOVID-19Multiplex analysisTh1 CellsSerology responsechemistryImmunoglobulin GImmunologybiology.proteinbusinessClinical Microbiology and Infection
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Role of HLA-B α-3 domain amino acid position 194 in HIV disease progression

2013

HLA class I molecules play a role in the regulation of innate immune response. Therefore, the interaction of HLA class I molecules with different activating and inhibitory receptors leads to balancing the immune response. Among the different family of receptors, NK receptors KIR3DL1/S1 and LIR1, play a major role. Aim of this study was to evaluate the role of amino acid polymorphic positions of HLA class I molecules interacting with NK receptors in HIV progression. In order to minimize the influence of viral variability, a cohort of children with a nosocomial monophyletic HIV-1 infection from the Benghazi Children Hospital has been evaluated. To assess the role of single amino acid position…

Models MolecularGene ExpressionKIR3DS1HIV InfectionsPeptide bindingLeukocyte Immunoglobulin-like Receptor B1ModelsImmunologicReceptorsInnateReceptors ImmunologicChildReceptorGeneticschemistry.chemical_classificationCross Infectioneducation.field_of_studyReceptors KIR3DL1Polymorphism Genetic; Models Molecular; Humans; Disease Progression; Gene Expression; HLA-B Antigens; Immunity Innate; Child; Receptors KIR3DL1; Protein Binding; HIV-1; Binding Sites; Receptors KIR3DS1; Receptors Immunologic; HIV Infections; Antigens CD; Protein Structure Tertiary; Signal Transduction; Amino Acid Substitution; Cross InfectionHLA-BCDAmino acidDisease ProgressionKIR3DL1Protein BindingSignal TransductionReceptors KIR3DS1Protein StructureImmunologyPopulationHuman leukocyte antigenBiologyGeneticKIR3DL1Antigens CDHumansPolymorphismAntigenseducationMolecular BiologySettore MED/04 - Patologia GeneralePolymorphism GeneticBinding SitesInnate immune systemImmunityMolecularImmunity InnateProtein Structure TertiaryAmino Acid SubstitutionchemistryHLA-B AntigensImmunologyHIV-1TertiaryMolecular Immunology
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Role of individual's T cell immunome in controlling HIV-1 progression

2014

Viral and host factors can influence HIV-1 progression, among them human leucocyte antigen (HLA) has shown the strongest effect. However, studies on the functional contribution of HLA in controlling HIV progression toward AIDS are limited by multiple issues, including the viral strain variability within the study subjects. In this study, in a cohort of children infected with a monophyletic strain (CRF02_AG) during an outbreak, we evaluated the HIV-1 Gag, Vif, Vpr, Tat and hepatitis C virus E1/E2 (as control) proteins circulating in a cohort for the capability to be presented by the HLA molecules in the same population. A total of 70 Non-progressors and 37 Progressors to AIDS were evaluated.…

MalevirusesHepatitis C virusImmunologyPopulationHIV InfectionsHuman leukocyte antigenBiologymedicine.disease_causeMajor histocompatibility complexgag Gene Products Human Immunodeficiency VirusEpitopeAntigenHLA AntigensT-Lymphocyte SubsetsConsensus SequencemedicineHumansImmunology and AllergyAmino Acid SequenceChildeducationAllelesPhylogenySettore MED/04 - Patologia Generaleeducation.field_of_studyHistocompatibility TestingSettore BIO/12Original ArticlesViral LoadGroup-specific antigenVirologyCD4 Lymphocyte CountPhenotypeChild PreschoolImmunologyDisease ProgressionHIV-1biology.proteinSettore ING-INF/06 - Bioingegneria Elettronica e InformaticaFemaleErratumSequence AlignmentViral load
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