0000000000767502
AUTHOR
Juan B. Peña
Fish tolerance to organophosphate-induced oxidative stress is dependent on the glutathione metabolism and enhanced by N-acetylcysteine
Abstract Dichlorvos (2,2-dichlorovinyl dimethyl phosphate, DDVP) is an organophosphorus (OP) insecticide and acaricide extensively used to treat external parasitic infections of farmed fish. In previous studies we have demonstrated the importance of the glutathione (GSH) metabolism in the resistance of the European eel ( Anguilla anguilla L.) to thiocarbamate herbicides. The present work studied the effects of the antioxidant and glutathione pro-drug N -acetyl- l -cysteine (NAC) on the survival of a natural population of A. anguilla exposed to a lethal concentration of dichlorvos, focusing on the glutathione metabolism and the enzyme activities of acetylcholinesterase (AChE) and caspase-3 a…
Filling gaps: closing the life cycle of the endangered Mediterranean limpet Patella ferruginea Gmelin, 1791 (Gastropoda, Patellidae)
Several reproductive issues and the larval development of the ferruginous limpet, Patella ferruginea Gmelin, 1791, an endangered species endemic from the western Mediterranean Sea, were studied to fill gaps in the knowledge of its life cycle. Average diameter of mature oocytes was 141.83 μm and mean oocyte density in the ovary was 283,800 oocytes/gram. No significant correlations were found between both oocyte diameter or density and female shell length. Female fecundity (number of oocytes per gonad) was significantly correlated with shell length and varied between 189,200 oocytes in a 40.0 mm female and 5,019,200 oocytes in an 86.4 mm female. However, there was considerable variability, in…
Allozyme variation in populations of scallops, Pecten jacobaeus (L.) and P. maximus (L.) (Bivalvia: Pectinidae), across the Almeria–Oran front
Abstract The scallops Pecten jacobaeus and P. maximus have been traditionally considered as different species which shared a common ancestor some 5 Ma ago. This view has been challenged by recent reports of genetic distances based on allozymes and mitochondrial DNA which are incompatible with such a long period of isolation and are more typical of conspecific populations. To explain the slight allozyme differentiation opposed to a clear morphological distinctiveness, two main hypotheses have been proposed. One hypothesis views interpopulation allozyme differentiation as an outcome of simple population genetic processes such isolation by distance. The other considers that the intertaxa alloz…
Increased recovery of brain acetylcholinesterase activity in dichlorvos-intoxicated European eels Anguilla anguilla by bath treatment with N-acetylcysteine
Organophosphate (OP) pesticides are widely used as antiparasitic chemicals in finfish aquaculture. However, current antidotes cannot be applied to treat intoxicated fish. We showed in previous studies the importance of glutathione (GSH) metabolism in pesticide resistance of the European eel Anguilla anguilla L. The present work studied the effects of the antioxidant and glutathione pro-drug N-acetyl-l-cysteine (NAC) on the recovery of European eels exposed for 96 h to a sublethal concentration (0.17 mg l^-1; 20% of its 96 h LC50) of the OP pesticide dichlorvos (2,2-dichlorovinyl dimethyl phosphate; DDVP). This insecticide and acaricide decreased muscular GSH content and increased oxidised g…
Role of glutathione in Thiobencarb resistance in the European eel Anguilla anguilla.
Glutathione-dependent defense against xenobiotic toxicity is a multifaceted phenomenon that has been well characterized in mammals. In the present study, eels of species Anguilla anguilla were exposed to 15 ppm of the herbicide thiobencarb (S-4-chlorobenzyl diethylthiocarbamate) for 96 h. Eels exposed to the pesticide were grouped in 24-h intervals according to their time of death, while surviving intoxicated eels constituted another group (live animals). Glutathione content (GSx, GSH, GSSG) was determined in liver and muscle tissues of the dead and live (intoxicated) animals and compared to control values (nonexposed eels). The fish that died before 96 h of exposure were considered suscept…