Genetic manipulation of spiramycin-producing strain Streptomyces ambofaciens ATCC 23877 by φC31 Att/Int system-based vectors results in a significant…

The genus Streptomyces produces about two-thirds of naturally occurring antibiotics and many other biologically active secondary metabolites. The genetic manipulation of Streptomycetes is often labor and time intensive due to their large genome and complex development. Much progress has been made to develop gene transfer methods useful to construct antibiotic-producing strains with improved properties. The φC31 Att/Int system is an integration system that has been widely used to produce stable recombinants in Actinomycetes and its integration site is an attB site located in a pirin-like gene (pirA) of the bacterial chromosome. In this study we demonstrate that the integration of φC31 DNA in…

Propagule dispersal and larval patch cohesiveness in a Mediterranean coastal fish

2016

The assessment of fish dispersal is fundamental for both conservation and management of fishery resources as it provides crucial information for the establishment of more effective marine protected areas (MPAs) and networks of MPAs. In this study, we investigated the elemental composition of otoliths in early life stages of the saddled sea bream Oblada melanura (Linnaeus, 1758) (Perciformes: Sparidae) in order to obtain information on its propagule (egg and larva) dispersal in the south-western Mediterranean Sea. Specifically, using pre-settlement individuals we investigated (1) larval patch cohesiveness during the last phase of larval life; and with early post-settlement individuals we inv…