0000000000774434

AUTHOR

Rainer Klocke

showing 2 related works from this author

Long-term expression of differentiated functions in hepatocytes cultured in three-dimensional collagen matrix.

1998

Hepatocytes entrapped in collagen gel and cultured in serum-free conditions survived longer than cells cultured on plastic (5 days vs. 3 weeks), showed fewer signs of early cell senescence (no increase in c-fos oncoprotein expression), and maintained the expression of differentiated hepatic metabolic functions over a longer period of time. Cells cultured in collagen gels retained their ability to respond to hormones. The insulin-stimulated glycogen synthesis rate remained fairly constant during 18 days in culture (between 5.4 +/- 0.37 and 9 +/- 2.7 nmol glucose/h/microg DNA). Collagen-cultured hepatocytes recovered glycogen stores to levels similar to those found in liver, or in hepatocytes…

Malemedicine.medical_specialtyPhysiologyCellular differentiationClinical BiochemistryCell Culture TechniquesIsozymeCulture Media Serum-FreeRats Sprague-Dawleychemistry.chemical_compoundCytochrome P-450 Enzyme SystemInternal medicinemedicineAnimalsInsulinUreaRNA MessengerEnzyme inducerGlycogen synthaseBiotransformationCells CulturedbiologyGlycogenReverse Transcriptase Polymerase Chain ReactionGenes fosCell DifferentiationCell BiologyGlutathioneMolecular biologyExtracellular MatrixLiver GlycogenRatsIsoenzymesEndocrinologychemistryGene Expression RegulationLiverPharmaceutical PreparationsCell cultureEnzyme InductionMethylcholanthrenebiology.proteinMicrosomes LiverHepatocytesCollagenProto-Oncogene Proteins c-fosTranscription Factors
researchProduct

Constitutive and inducible expression of CYP enzymes in immortal hepatocytes derived from SV40 transgenic mice

2003

1. The expression of liver-specific transcription factors and cytochrome P450 (CYP) enzymes have been studied in three new hepatocyte-like cell lines derived from SV Delta 202 transgenic mice: AMH-Delta 202 (adult mouse hepatocytes), TAMH-Delta 202 (tumour-derived adult mouse hepatocytes) and NMH-Delta 202 (newborn mouse hepatocytes). 2. mRNA levels of liver-enriched transcription factors such as D-element binding protein (DBP), liver-enriched transcription activating protein (LAP) and the hepatic nuclear factors (HNF) 1, 2 and 3 in all Delta 202 transgenic hepatocyte lines were similar to those in the wild-type liver and in primary mouse hepatocytes. 3. Analysis of basal CYP activities and…

Genetically modified mouseHealth Toxicology and MutagenesisTransgeneGene ExpressionMice TransgenicBiologyHydroxylationToxicologyBiochemistryDexamethasoneCell LineMiceCytochrome P-450 Enzyme SystemGene expressionmedicineAnimalsTestosteroneRNA MessengerTranscription factorPharmacologyEthanolCytochrome P450General MedicineCYP2E1Molecular biologymedicine.anatomical_structureLiverCell cultureEnzyme InductionPhenobarbitalHepatocyteHepatocytesbiology.proteinRifampinMethylcholanthreneTranscription FactorsXenobiotica
researchProduct