0000000000779197

AUTHOR

Elina Ikonen

0000-0001-8382-1135

showing 3 related works from this author

Cholesterol Dependence of Collagen and Echovirus 1 Trafficking along the Novel α2β1 Integrin Internalization Pathway

2013

We have previously shown that soluble collagen and a human pathogen, echovirus 1 (EV1) cluster α2β1 integrin on the plasma membrane and cause their internalization into cytoplasmic endosomes. Here we show that cholesterol plays a major role not only in the uptake of α2β1 integrin and its ligands but also in the formation of α2 integrin-specific multivesicular bodies (α2-MVBs) and virus infection. EV1 infection and α2β1 integrin internalization were totally halted by low amounts of the cholesterol-aggregating drugs filipin or nystatin. Inhibition of cholesterol synthesis and accumulation of lanosterol after ketoconazole treatment inhibited uptake of collagen, virus and clustered integrin, an…

IntegrinsNystatinFluorescent Antibody TechniqueBiochemistryCollagen receptorchemistry.chemical_compoundBINDINGMolecular Cell BiologyInternalizationLipid raftREQUIRESmedia_common0303 health sciencesMicroscopy ConfocalMultidisciplinarybiologyQRIMMUNODEFICIENCY-VIRUS TYPE-1RNA REPLICATIONCellular StructuresExtracellular MatrixEnterovirus B Human3. Good healthCell biologyProtein TransportCholesterolENTRYCytochemistryMedicineMembranes and Sortinglipids (amino acids peptides and proteins)CollagenIntegrin alpha2beta1Research ArticleSignal TransductionViral EntryEndosomeSciencemedia_common.quotation_subjecteducationIntegrinLOW-DENSITY-LIPOPROTEINMicrobiologyFilipinClathrinGPI-ANCHORED PROTEINS03 medical and health sciencesVirologyCell Line TumorCell AdhesionHumansFilipinBiology030304 developmental biology030306 microbiologyCell MembraneVirus Uncoatingta1182TRANSPORTLIPID RAFTSMicroscopy ElectronSubcellular Organelleschemistrybiology.protein3111 BiomedicineChromatography Thin LayerCELL-MEMBRANESViral Transmission and InfectionPLoS ONE
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Mobilization of late-endosomal cholesterol is inhibited by Rab guanine nucleotide dissociation inhibitor

2000

AbstractCholesterol entering cells in low-density lipoproteins (LDL) via receptor-mediated endocytosis is transported to organelles of the late endocytic pathway for degradation of the lipoprotein particles. The fate of the free cholesterol released remains poorly understood, however. Recent observations suggest that late-endosomal cholesterol sequestration is regulated by the dynamics of lysobisphosphatidic acid (LBPA)-rich membranes [1]. Genetic studies have pinpointed a protein, Niemann–Pick C-1 (NPC-1), that is required for the mobilization of late-endosomal/lysosomal cholesterol by an unknown mechanism [2]. Here, we report the removal of accumulated cholesterol by overexpression of the…

HydrolasesEndosomeEndocytic cycleEndosomesCholesterol 7 alpha-hydroxylaseGeneral Biochemistry Genetics and Molecular BiologyCell Line03 medical and health scienceschemistry.chemical_compound0302 clinical medicineHumansGuanine Nucleotide Dissociation Inhibitors030304 developmental biologyNiemann-Pick Diseases0303 health sciencesbiologyAgricultural and Biological Sciences(all)CholesterolBiochemistry Genetics and Molecular Biology(all)Reverse cholesterol transportCholesterol LDLEndocytosisRecombinant ProteinsCell biologyCholesterolchemistryBiochemistryHMG-CoA reductasebiology.proteinMonoglycerideslipids (amino acids peptides and proteins)RabLysophospholipidsLysosomesGeneral Agricultural and Biological Sciences030217 neurology & neurosurgeryLipoproteinCurrent Biology
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Cholesterol Dependence of Collagen and Echovirus 1 Trafficking along the Novel alpha2beta1 Integrin Internalization Pathway

2013

We have previously shown that soluble collagen and a human pathogen, echovirus 1 (EV1) cluster α2β1 integrin on the plasma membrane and cause their internalization into cytoplasmic endosomes. Here we show that cholesterol plays a major role not only in the uptake of α2β1 integrin and its ligands but also in the formation of α2 integrin-specific multivesicular bodies (α2-MVBs) and virus infection. EV1 infection and α2β1 integrin internalization were totally halted by low amounts of the cholesterol-aggregating drugs filipin or nystatin. Inhibition of cholesterol synthesis and accumulation of lanosterol after ketoconazole treatment inhibited uptake of collagen, virus and clustered integrin, an…

echovirus 1integrinkolesterolilipids (amino acids peptides and proteins)integriini
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