showing 5 related works from this author
Co-administration of human MSC overexpressing HIF-1α increases human CD34+ cell engraftment in vivo
2021
Abstract Background Poor graft function or graft failure after allogeneic stem cell transplantation is an unmet medical need, in which mesenchymal stromal cells (MSC) constitute an attractive potential therapeutic approach. Hypoxia-inducible factor-1α (HIF-1α) overexpression in MSC (HIF-MSC) potentiates the angiogenic and immunomodulatory properties of these cells, so we hypothesized that co-transplantation of MSC-HIF with CD34+ human cord blood cells would also enhance hematopoietic stem cell engraftment and function both in vitro and in vivo. Methods Human MSC were obtained from dental pulp. Lentiviral overexpression of HIF-1α was performed transducing cells with pWPI-green fluorescent pr…
Additional file 1 of Co-administration of human MSC overexpressing HIF-1�� increases human CD34+ cell engraftment in vivo
2021
Additional file 1. Additional methods.
Additional file 3 of Co-administration of human MSC overexpressing HIF-1�� increases human CD34+ cell engraftment in vivo
2021
Additional file 3. Figure S2. Immunophenotypic analysis of MSC WT and HIF-MSC. Histogram of the different surface molecules analyzed by flow cytometry. MSC WT: MSC wild type; HIF-MSC: MSC overexpressing HIF-1��
Additional file 4 of Co-administration of human MSC overexpressing HIF-1�� increases human CD34+ cell engraftment in vivo
2021
Additional file 4. Figure S3. Analysis of human hematopoietic engraftment in spleen. Human hematopoietic engraftment was evaluated by flow cytometry after xenotrasplantation in NOD/SCID mice. The percentage of human CD45+ cells (donor chimerism) was analyzed in total spleen samples 4 weeks after transplantation. Mice were transplanted intravenously with CD34+ cells alone or co-transplanted with MSC WT or HIF-MSC, that were administered in the right femur. Data are represented as mean of 8 experiments for mice transplanted with CD34+ cells alone, 13 experiments for mice co-transplanted with CD34+ cells and WT MSC and 15 experiments for mice transplanted with CD34+ cells and HIF-MSC. p:n.s me…
Additional file 2 of Co-administration of human MSC overexpressing HIF-1�� increases human CD34+ cell engraftment in vivo
2021
Additional file 2. Figure S1. Transduction efficiency of MSC. Histogram of a representative sample, with transfection efficiency >90%. MSC WT: MSC wild type; HIF-MSC: MSC overexpressing HIF-1��; GFP: Green Fluorescent Protein