0000000000795734

AUTHOR

Sandra L. Uratsu

showing 4 related works from this author

Transcriptome profiling of citrus fruit response to huanglongbing disease.

2010

Huanglongbing (HLB) or "citrus greening" is the most destructive citrus disease worldwide. In this work, we studied host responses of citrus to infection with Candidatus Liberibacter asiaticus (CaLas) using next-generation sequencing technologies. A deep mRNA profile was obtained from peel of healthy and HLB- affected fruit. It was followed by pathway and protein-protein network analysis and quantitative real time PCR analysis of highly regulated genes. We identified differentially regulated pathways and constructed networks that provide a deep insight into the metabolism of affected fruit. Data mining revealed that HLB enhanced transcription of genes involved in the light reactions of phot…

CitrusProtein FoldingGene Identification and Analysislcsh:MedicinePlant ScienceTranscriptomechemistry.chemical_compoundRNA interferencePlant Growth RegulatorsGene Expression Regulation PlantModelsGene expressionPlant Genomics2.1 Biological and endogenous factorsPhotosynthesisAetiologylcsh:SciencePlant Growth and DevelopmentPlant PestsMultidisciplinaryProtein StabilityJasmonic acidfood and beveragesHigh-Throughput Nucleotide SequencingAgriculturePlantsCell biologyCarbohydrate MetabolismResearch ArticleSignal TransductionGeneral Science & TechnologyPlant PathogensProtein degradationBiologyModels BiologicalFruitsMolecular GeneticsRhizobiaceaeSettore AGR/07 - Genetica AgrariaHeat shock proteinBotanyGeneticsGene RegulationGene NetworksBiologyTranscription factorPlant DiseasesAnalysis of VarianceGene Expression Profilinglcsh:RCitrus HLB next-generation sequencing candidatus liberibacterComputational BiologyPlantPlant PathologyBiologicalWRKY protein domainGene expression profilingchemistryGene Expression Regulationlcsh:QGene expressionGene FunctionTranscriptomeTranscription Factors
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Transcriptome and metabolome analysis of citrus fruit to elucidate puffing disorder.

2014

a b s t r a c t A systems-level analysis reveals details of molecular mechanisms underlying puffing disorder in Citrus fruit. Flavedo, albedo and juice sac tissues of normal fruits and fruits displaying symptoms of puffing disorder were studied using metabolomics at three developmental stages. Microarrays were used to compare normal and puffed fruits for each of the three tissues. A protein-protein interaction network inferred from previous work on Arabidopsis identified hub proteins whose transcripts show significant changes in expression. Glycolysis, the backbone of primary metabolism, appeared to be severely affected by the disorder, based on both transcriptomic and metabolomic results. …

CitrusPlant ScienceBiologyTranscriptomechemistry.chemical_compoundMetabolomicsPlant Growth RegulatorsArabidopsisGeneticsMetabolomeBrassinosteroidMetabolomicsProtein Interaction MapsAbscisic acidOligonucleotide Array Sequence AnalysisPlant DiseasesAlbedo breakdown Citrus Fruit disorder Metabolomics Puffing TranscriptomicsGene Expression Profilingfood and beveragesGeneral Medicinebiology.organism_classificationchemistryBiochemistryFruitGibberellinCitric acidAgronomy and Crop ScienceSignal TransductionTranscription FactorsPlant science : an international journal of experimental plant biology
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Gene regulation in parthenocarpic tomato fruit.

2009

Parthenocarpy is potentially a desirable trait for many commercially grown fruits if undesirable changes to structure, flavour, or nutrition can be avoided. Parthenocarpic transgenic tomato plants (cv MicroTom) were obtained by the regulation of genes for auxin synthesis (iaaM) or responsiveness (rolB) driven by DefH9 or the INNER NO OUTER (INO) promoter from Arabidopsis thaliana. Fruits at a breaker stage were analysed at a transcriptomic and metabolomic level using microarrays, real-time reverse transcription-polymerase chain reaction (RT-PCR) and a Pegasus III TOF (time of flight) mass spectrometer. Although differences were observed in the shape of fully ripe fruits, no clear correlatio…

0106 biological sciencesPhysiologyParthenogenesisPlant Biologyseedless fruitPlant SciencetomatoParthenocarpy01 natural sciencesSolanum lycopersicumGene Expression Regulation PlantGene expressionArabidopsis thalianaHormone metabolismPlant Proteins2. Zero hungerchemistry.chemical_classification0303 health sciencesbiologyfood and beveragesRipeningPlantsPlants Genetically ModifiedResearch PapersBiochemistryMetabolomeBiotechnologyCrop and Pasture ProductionINOPlant Biology & Botanyfruit ripeningGenetically Modified03 medical and health sciencesparthenocarpicAuxinBotanyGeneticsGenetically modified tomatoLycopersicon esculentum030304 developmental biologyNutritionfruit quality fruit ripening INO parthenocarpic seedless fruit tomato.Arabidopsis Proteinsfungifruit qualityPlantbiology.organism_classificationSeedless fruitchemistryGene Expression RegulationFruit010606 plant biology & botanyTranscription Factors
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Gene regulatory networks elucidating Huanglongbing disease mechanisms

2013

Next-generation sequencing was exploited to gain deeper insight into the response to infection by Candidatus liberibacter asiaticus (CaLas), especially the immune disregulation and metabolic dysfunction caused by source-sink disruption. Previous fruit transcriptome data were compared with additional RNA-Seq data in three tissues: immature fruit, and young and mature leaves. Four categories of orchard trees were studied: symptomatic, asymptomatic, apparently healthy, and healthy. Principal component analysis found distinct expression patterns between immature and mature fruits and leaf samples for all four categories of trees. A predicted protein - protein interaction network identified HLB-…

CitrusGeneral Science & TechnologyGene regulatory networklcsh:MedicineBiologyCarbohydrate metabolismPolymerase Chain ReactionTranscriptomeImmune systemDownregulation and upregulationGene expressionNoneGenetics2.1 Biological and endogenous factorsGene Regulatory NetworksAetiologylcsh:ScienceGeneNutritionPlant DiseasesGeneticsMultidisciplinarylcsh:Rfood and beveragesInvertaselcsh:QTranscriptomeResearch Article
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