0000000000795911

AUTHOR

Juan Carlos Montesinos

showing 3 related works from this author

Arabidopsis p24δ5 and p24δ9 facilitate Coat Protein I-dependent transport of the K/HDEL receptor ERD2 from the Golgi to the endoplasmic reticulum.

2014

The p24 proteins belong to a family of type I membrane proteins which cycle between the endoplasmic reticulum (ER) and Golgi via coat protein I (COPI) and COPII vesicles. Current nomenclature classifies them into four subfamilies, although plant p24 proteins belong to either the p24β or the p24δ subfamilies. Here, we show that Arabidopsis p24δ5/δ9 and HDEL ligands shift the steady-state distribution of the K/HDEL receptor ERD2 from the Golgi to the ER. We also show that p24δ5/δ9 interact directly with ERD2. This interaction requires the Golgi dynamics (GOLD) domain in p24δ5 and is much higher at acidic than at neutral pH, consistent with both proteins interacting at the cis-Golgi. In additi…

Receptors PeptideArabidopsisGolgi ApparatusPlant ScienceBiologyEndoplasmic ReticulumCoat Protein Complex Isymbols.namesakeGeneticsAnimalsSecretionCOPIIVesicular-tubular clusterArabidopsis ProteinsEndoplasmic reticulumMembrane ProteinsCell BiologyCOPIGolgi apparatusCell biologyTransport proteinDNA-Binding ProteinsProtein TransportMembrane proteinsymbolsTranscription FactorsThe Plant journal : for cell and molecular biology
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Putative p24 complexes in Arabidopsis contain members of the delta and beta subfamilies and cycle in the early secretory pathway

2013

p24 proteins are a family of type I membrane proteins localized to compartments of the early secretory pathway and to coat protein I (COPI)- and COPII-coated vesicles. They can be classified, by sequence homology, into four subfamilies, named p24α, p24β, p24γ, and p24δ. In contrast to animals and fungi, plants contain only members of the p24β and p24δ subfamilies, the latter probably including two different subclasses. It has previously been shown that transiently expressed red fluorescent protein (RFP)-p24δ5 (p24δ1 subclass) localizes to the endoplasmic reticulum (ER) at steady state as a consequence of highly efficient COPI-based recycling from the Golgi apparatus. It is now shown that tr…

PhysiologyArabidopsisGolgi ApparatusPlant ScienceEndoplasmic ReticulumGreen fluorescent proteinsymbols.namesakeArabidopsisImmunoprecipitationER–Golgi transportcoat protein II (COPII)Secretory pathwayMicroscopy ConfocalSecretory PathwaybiologyArabidopsis ProteinsEndoplasmic reticulumcoat protein I (COPI)COPIImmunogold labellingGolgi apparatussecretory pathway.biology.organism_classificationImmunohistochemistryCell biologyMicroscopy Electronp24 proteinsMembrane proteinsymbolsResearch PaperPlasmidsJournal of Experimental Botany
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Coupled transport of Arabidopsis p24 proteins at the ER–Golgi interface

2012

p24 proteins are a family of type I membrane proteins localized to compartments of the early secretory pathway and to coat protein I (COPI)- and COPII-coated vesicles. They can be classified, by sequence homology, into four subfamilies, named p24α, p24β, p24γ, and p24δ. In contrast to animals and fungi, plants contain only members of the p24β and p24δ subfamilies. It has previously been shown that transiently expressed red fluorescent protein (RFP)–p24δ5 localizes to the endoplasmic reticulum (ER) as a consequence of highly efficient COPI-based recycling from the Golgi apparatus. Using specific antibodies, endogenous p24δ5 has now been localized to the ER and p24β2 to the Golgi apparatus in…

PhysiologyMolecular Sequence DataArabidopsisGolgi ApparatusPlant ScienceBiologyEndoplasmic Reticulumcoat protein (COP) IIcoat protein (COP) Isymbols.namesakeAmino Acid SequenceER–Golgi transportCOPIISecretory pathwayArabidopsis ProteinsVesicular-tubular clusterEndoplasmic reticulumMembrane ProteinsCOPIGolgi apparatussecretory pathwayTransport proteinCell biologyProtein TransportSecretory proteinp24 proteinssymbolsProtein BindingResearch PaperJournal of Experimental Botany
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