0000000000798226
AUTHOR
Katarzyna Grata
Bacillus subtilis BS-2 and Peppermint Oil as Biocontrol Agents Against Botrytis cinerea
Abstract The purpose of this study was to assess the activity of Bacillus subtilis BS-2 and peppermint oil against Botrytis cinerea. In this study parameters such as the age and the density of the bacterial culture and the incubation temperature were taken into consideration. Furthermore, the cellulolytic activity of the bacterium was determined. The effect of peppermint oil was evaluated at a concentration range of 0.5-4.0 %. The research was conducted with a dual culture plate method. The influence of B. subtilis BS-2 and peppermint oil on the growth of B. cinerea was evaluated based on the growth rate index. It was noted that the bacterial culture occurred at an initial density of OD 560…
Nature-Inspired Effects of Naturally Occurring Trace Element-Doped Hydroxyapatite Combined with Surface Interactions of Mineral-Apatite Single Crystals on Human Fibroblast Behavior †
Innovative engineering design for biologically active hydroxyapatites requires enhancing both mechanical and physical properties, along with biocompatibility, by doping with appropriate chemical elements. Herein, the purpose of this investigation was to evaluate and elucidate the model of naturally occurring hydroxyapatite and the effects of doped trace elements on the function of normal human fibroblasts, representing the main cells of connective tissues. The substrates applied (geological apatites with hexagonal prismatic crystal habit originated from Slyudyanka, Lake Baikal, Russia (GAp) and from Imilchil, The Atlas Mountains, Morocco (YAp)) were prepared from mineral natural apatite wit…
Determining Cellulolytic Activity of Microorganisms
Abstract Decomposition of cellulose to glucose requires complex cooperation of glycoside hydrolase enzymes. As a result of glycoside β-1,4 bonds hydrolysis, shorter chains of cellulose, oligodextrin, cellobiose and glucose are created. A number of bacteria and fungi demonstrate the capacity to degrade cellulose. Their activity can be assessed with the use of qualitative and quantitative methods. Qualitative methods with the use of e.g. Congo red, are used in screening studies, however, they do not provide information about the quantity of the produced enzyme. Spectrophotometric methods are more accurate and they measure the quantities of reducing sugars with the use of appropriate substrate…
The Influence of Sewage Sludge and Fly Ash Fertilization on the Total Number of Bacteria (TNB) and Bradyrhizobium Species in Soybean Agroecosystem
The aim of this study was to determine the impact of different chemical properties in soil, including changes in magnetic susceptibility and the impact of humic substances from sewage sludge and fly ash on the microbial community in the soybean agroecosystem. A field experiment was carried out using the random plot method on Cambisol with a texture of silt loam. The experiment consisted of 24 plots (six treatments and four replications) using different dozes of sewage sludge and fly ash. The following physicochemical and chemical analyses were performed in the soil samples: pH in KCl, conductivity (λ), total content of heavy metal, magnetic measurements and fractional composition of …