0000000000798498

AUTHOR

Rodrigo Rocha Latado

showing 2 related works from this author

Gametic and somatic embryogenesis through in vitro anther culture of different Citrus genotypes

2015

Abstract: In vitro tissue culture represents a useful technique for advancing Citrus breeding and propagation. Among in vitro regeneration systems, anther culture is commonly used to produce haploids and doubled haploids for a fast-track producing homozygous lines, in comparison with the traditional self-pollination approach, which involves several generations of selfing. In addition, anthers culture can produce somatic embryos that can also be used for clonal propagation. In this study, two thermal shocks were applied to the anthers of six Citrus genotypes (two clementine and four sweet oranges), just after they were put in culture. The response obtained was different depending on the geno…

0106 biological sciences0301 basic medicineAnther cultureSomatic embryogenesiscytofluorometryEvolutionStamenPlant ScienceBiology01 natural sciences03 medical and health sciencesTissue cultureBehavior and Systematicsmicrospore embryogenesiBotanyCultivarEcology Evolution Behavior and SystematicsEcologyfungiAnther culture; cytofluorometry; homozygosity; microspore embryogenesis; ploidy level; Ecology Evolution Behavior and Systematics; Plant Sciencefood and beveragesSelfingEmbryoploidy levelEcology Evolution Behavior and SystematicSettore AGR/03 - Arboricoltura Generale E Coltivazioni Arboree030104 developmental biologymicrospore embryogenesisPloidyhomozygosityClementine010606 plant biology & botany
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In vitro anther culture of sweet orange (Citrus sinensis L. Osbeck) genotypes and of a C. clementina × C. sinensis 'Hamlin' hybrid

2014

Citrus, and particularly sweet oranges, are very recalcitrant to anther culture. In this paper it was evaluated for the first time the response of 27 genotypes of Citrus sinensis and of one hybrid C. clementina × C. sinensis, to in vitro anther culture. Ten genotypes of sweet oranges showed embryogenic callus induction, mostly blood sweet oranges genotypes, such as Tarocco, Moro and Sanguinelli. In vitro microspore developmental switches from the gamethophytic to the sporophytic pathway were shown by DAPI staining in microspores of these responsive genotypes, after 10 months in culture. However, microsatellite marker analyses showed that these calli were heterozygous. The flow-cytometric an…

Molecular profileTri haploidfungiStamenCitrufood and beveragesEmbryoOrange (colour)BiologyHorticultureCalliSettore AGR/03 - Arboricoltura Generale E Coltivazioni ArboreeMicrosporeCallusBotanyMicrosatelliteHaploidPloidyMicrosporogenesiCitrus × sinensis
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