Vaccination against HPV-Associated Neoplasias

Neoplasias associated with anogenital human papilloma viruses (HPV) are characterised by high patient morbidity and mortality and by appreciable limitations in the patientʼs quality of life. Each year 530,000 women worldwide and 4800 women in Germany develop cervical cancer 1, 2. Biomolecular and epidemiological studies carried out in the past 25 years have demonstrated causal link between persisting infections with HPV 16 and HPV 18 and at least 11 other so-called high-risk HPVs (HR-HPVs) and the development of cervical cancer and its precursor lesions (so-called dysplasias or, respectively, cervical intraepithelial neoplasias – CIN). HPV 16, HPV 18 and other HR-HPVs are also the causes of…

Genome-Wide DNA Methylation Profiling in Early Stage I Lung Adenocarcinoma Reveals Predictive Aberrant Methylation in the Promoter Region of the Long Noncoding RNA PLUT : An Exploratory Study

Introduction: Surgical procedure is the treatment of choice in early stage I lung adenocarcinoma. However, a considerable number of patients experience recurrence within the first 2 years after complete resection. Suitable prognostic biomarkers that identify patients at high risk of recurrence (who may probably benefit from adjuvant treatment) are still not available. This study aimed at identifying methylation markers for early recurrence that may become important tools for the development of new treatment modalities. Methods: Genome-wide DNA methylation profiling was performed on 30 stage I lung adenocarcinomas, comparing 14 patients with early metastatic recurrence with 16 patients with …

Impfprävention HPV-assoziierter Neoplasien : Empfehlungen aus der aktuellen S3-Leitlinie des HPV-Management-Forums der Paul-Ehrlich-Gesellschaft für Chemotherapie e. V. – AWMF Leitlinien-Register Nr. 082-002 (Kurzfassung), gültig bis 31.12.2018

Separation studies of amino acids, proteins and enzymes on bonded 1,2-dihydroxy-, 1,2-hydroxylamino-and amino silica packings

1,2-dihydroxy-3-propoxypropyl (HPPS), 1-amino-2-hydroxy-3-propoxypropyl (AHPS) and 1-aminoethyl-3-aminopropyl (AEAPS) silica were synthesized by means of both a surface modification procedure (I) and a bulk modification procedure (II). Method (I) gave a surface concentration, α, of functional groups of 2–3 μmole/m2, whereas method (II) gave values up to 5 μmole/m2. Retention times, peak asymmetries and plate heights of thiamine and ascorbic acid eluted with aqueous buffer solutions ranging from pH 5.3 to 9.2 gave only a±5% variation over periods of 12 hours and more. The recoveries of selected enzymes and proteines examined under static and dynamic conditions were between 60% and 100% depen…

Synthesis and properties of chemically modified dihydroxy-, hydroxyamino- and amino-functional silica packings in adsorption chromatography

1,2-Dihydroxy-3-propoxypropyl (HPP), 1-amino-2-hydroxy-3-propoxypropyl (AHP) and 3-(2-aminoethylamino)propyl (AEAP) silica packings were synthesized by means of both a surface modification and a bulk modification procedure. In surface modification a complete monolayer of bonded groups could be achieved. The modifiers reacted with the silanol groups according to a bifunctional mechanism. Eluotropic series of solvents were established on these polar modified silicas referring to n-heptane having ɛ∘=0. The solvent parameter ratio of modified to bare silica was found to be constant over a wide range of solvent compositions as \(\varepsilon _{{\text{HPP - silica}}}^ \circ = 0.26\varepsilon _{{\t…