0000000000803370
AUTHOR
P. Vargas
The colonization history of Olea europaea L. in Macaronesia based on internal transcribed spacer 1 (ITS-1) sequences, randomly amplified polymorphic DNAs (RAPD), and intersimple sequence repeats (ISSR)
Phylogenetic relationships in the Olea europaea complex and the phylogeography of 24 populations of the Macaronesian olive (O. europaea ssp. cerasiformis) were assessed by using three molecular markers: nuclear ribosomal internal transcribed spacer 1 (ITS-1) sequences, randomly amplified polymorphic DNAs (RAPD), and intersimple sequence repeats (ISSR). Parsimony analysis of the ITS-1 sequences and Neighbour-joining (NJ) analyses of RAPD and ISSR banding variation revealed four major lineages in the O. europaea complex: (1) ssp. cuspidata; (2) ssp. cerasiformis from Madeira; (3) ssp. laperrinei; and (4) ssp. cerasiformis from the Canary Islands plus ssp. europaea. These results provide unequ…
Molecular fingerprinting evidence (ISSR, Inter-Simple Sequence Repeats) for a wild status of Olea europaea L. (Oleaceae) in the Eurosiberian North of the Iberian Peninsula
Summary Although the olive tree ( Olea europaea L.) is one of the best indicators of the Mediterranean region, there exist some isolated populations of this species in the Eurosiberian North of the Iberian Peninsula. To elucidate the wild or feral status of these populations, we studied a total of 46 populations of the Olea europaea complex. mat K sequences and Inter-Simple Sequence Repeats (ISSR) variation were analysed to infer relationships among the six subspecies of the Olea europaea complex and within O. europaea subsp. europaea. Based on mat K. sequences, populations of sspp. europaea (Mediterranean basin), cerasiformis (Madeira), guanchica (Canary Islands), maroccana (Agadir Mountai…