0000000000804815
AUTHOR
Catalano Caterina
IN-VITRO REGENERATION OF CALENDULA MARITIMA GUSS. (ASTERACEAE), A THREATENED PLANT ENDEMIC TO WESTERN SICILY
Calendula maritima is a critically endangered endemic plant of Western Sicily. Besides habitat destruction, the hybridization with the contiguous congener species C. fulgida is a major threat to its conservation. For this reason, seed-based propagation and seed storage are not appropriate for conservation purposes. In the present paper we describe a rapid and prolific in vitro plant regeneration method by direct organogenesis from leaves of C. maritima. Leaf explants were cultured on solid Murashige and Skoog (MS) medium in the presence of several plant growth regulator combinations. The best shoot multiplication rate (2.5 shoots/explant) was obtained on the medium containing 4.4 µM 6-benzy…
Cultivation of Dill (Anethum graveolens L.) with different row arrangements
Dill (Anethum graveolens L.) is an annual plant from Umbelliferae. Although in cookery also sprouts and tender leaves are used, the drug from Dill is represented by the scented fruits (“seeds”), largely used for flavouring conserved foods and liqueurs, but also by herbal and cosmetic industry. In this work, we present the results of a trial performed in Sicily, putting under comparison four row arrangements: CR30 (continuous rows 30 cm apart); CR60 (continuous rows 60 cm apart), CR90 (continuous rows 90 cm apart) and TR (twin rows with a distance of 30 cm inside and 60 cm between twin rows). Being constant the plant population on row (12 plants m-1), the plant density per unit area changed …
In-vitro regeneration of Calendula maritima Guss. (Asteraceae), a threatened plant endemic to Western Sicily
Calendula maritima is a critically endangered endemic plant of Western Sicily. Besides habitat destruction, the hybridization with the contiguous congener species C. fulgida is a major threat to its conservation. For this reason, seed-based propagation and seed storage are not appropriate for conservation purposes. In the present paper we describe a rapid and prolific In vitro plant regeneration method by direct organogenesis from leaves of C. maritima. Leaf explants were cultured on solid Murashige and Skoog (MS) medium in the presence of several plant growth regulator combinations. The best shoot multiplication rate (2.5 shoots/explant) was obtained on the medium containing 4.4 mu M 6-ben…