Putative high mobility group non-histone chromosomal proteins from pea (Pisum sativum)

1991

Abstract Three putative HMG proteins, 1P, 2P and 3P have been isolated from pea ( Pisum sativum L. cv. Lincoln) nuclei by extraction with either 5% perchloric acid or 0.35 M NaCl and purified by preparative electrophoresis. The amino acid analysis showed many of the typical features of the HMG proteins, although 1P and 2P possess a somewhat reduced content of acidic amino acids and 3P has less than 20% basic amino acids. Peptide mapping with Staphylococcus aureus V8 protease suggested that none of the proteins are proteolytic products of histone H1.