0000000000821674

AUTHOR

Nicolas Perrin

0000-0002-7756-6323

showing 2 related works from this author

Strong reproductive barriers in a narrow hybrid zone of West-Mediterranean green toads (Bufo viridis subgroup) with Plio-Pleistocene divergence

2010

Abstract Background One key question in evolutionary biology deals with the mode and rate at which reproductive isolation accumulates during allopatric speciation. Little is known about secondary contacts of recently diverged anuran species. Here we conduct a multi-locus field study to investigate a contact zone between two lineages of green toads with an estimated divergence time of 2.7 My, and report results from preliminary experimental crosses. Results The Sicilian endemic Bufo siculus and the Italian mainland-origin B. balearicus form a narrow hybrid zone east of Mt. Etna. Despite bidirectional mtDNA introgression over a ca. 40 km North-South cline, no F1 hybrids could be found, and nu…

Male0106 biological sciencesBufo viridis hybrid zone mtDNA microsatellitesGenotypeEvolutionPopulationAllopatric speciationSettore BIO/05 - ZoologiaZoologyIntrogressionBiologyDNA Mitochondrial010603 evolutionary biology01 natural sciencesEvolution Molecular03 medical and health sciencesHybrid zoneQH359-425AnimalsCluster AnalysiseducationCrosses GeneticPhylogenyEcology Evolution Behavior and Systematics030304 developmental biologyHybridLocal adaptationCell NucleusPrincipal Component Analysis0303 health scienceseducation.field_of_studyChimeraMediterranean RegionReproductionGenetic VariationBayes TheoremSequence Analysis DNAReproductive isolationCline (biology)BufonidaeIntronsGenetics PopulationEvolutionary biologyHybridization GeneticFemaleGenetic FitnessMicrosatellite RepeatsResearch ArticleBMC Evolutionary Biology
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New polymorphic microsatellite markers and development of mitotyping primers for West Mediterranean green toad species (Bufo viridis subgroup).

2009

We report new polymorphic microsatellites for three species of Palearctic green toads (Bufo viridis subgroup): 10 in B. balearicus and seven each in B. siculus and B. boulengeri. Diversity at these loci, measured for 27 B. balearicus, 23 B. siculus and 11 B. boulengeri, ranged from low to high (two to 10 alleles). Mitotyping primers, specific to the control region, which allow fast screening of parapatric Sicilian endemic B. siculus and Italian mainland-origin B. balearicus, were developed.

Bufo viridisZoologySiculusToadBiologyParapatric speciationPolymorphic microsatellitesbiology.animalBotanyGeneticsPolymorphic Microsatellite MarkerMicrosatelliteEcology Evolution Behavior and SystematicsBiotechnologyWest mediterraneanMolecular ecology resources
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