6533b85bfe1ef96bd12bb49a

RESEARCH PRODUCT

New polymorphic microsatellite markers and development of mitotyping primers for West Mediterranean green toad species (Bufo viridis subgroup).

Caroline ColliardAlessandra SiciliaNicolas PerrinCraig MoritzMatthias Stöck

subject

Bufo viridisZoologySiculusToadBiologyParapatric speciationPolymorphic microsatellitesbiology.animalBotanyGeneticsPolymorphic Microsatellite MarkerMicrosatelliteEcology Evolution Behavior and SystematicsBiotechnologyWest mediterranean

description

We report new polymorphic microsatellites for three species of Palearctic green toads (Bufo viridis subgroup): 10 in B. balearicus and seven each in B. siculus and B. boulengeri. Diversity at these loci, measured for 27 B. balearicus, 23 B. siculus and 11 B. boulengeri, ranged from low to high (two to 10 alleles). Mitotyping primers, specific to the control region, which allow fast screening of parapatric Sicilian endemic B. siculus and Italian mainland-origin B. balearicus, were developed.

10.1111/j.1755-0998.2009.02600.xhttps://pubmed.ncbi.nlm.nih.gov/21564854