serrano gene and NaCl perception

Gustatory information is considered to be important for animals to control behavior when they seek for food or partners. 60 members of gustatory receptor (Gr) genes have been identified in Drosophila.. However little is now about mechanisms of taste perception and transduction in response to stimuli. In order to identify new genes involved in gustation, we performed a genetic screen using Gal4-UAS system based on expression of the reporter gene in chemosensory organs and gustatory defects in larvae and adults. We identified the serrano (sano) gene, which encodes a 778 aa protein with a leucine zipper domain, a putative transmembrane domain, and putative bipartite nuclear localization signal…

serrano gene and NaCl perception in Drosophila

Résumé de poster présenté au congrès Neurofly à Manchester en Septembre 2010.; International audience

Desat expression in neural and non-neural tissues separately affects perception and emission of sex pheromones in Drosophila

Crosstalk between hypothalamic neurons and olfactory system: a tracing and anatomo-fuctional investigation in mouse

The histone acetyltransferase MOF activates hypothalamic polysialylation to prevent diet-induced obesity in mice

Overfeeding causes rapid synaptic remodeling in hypothalamus feeding circuits. Polysialylation of cell surface molecules is a key step in this neuronal rewiring and allows normalization of food intake. Here we examined the role of hypothalamic polysialylation in the long-term maintenance of body weight, and deciphered the molecular sequence underlying its nutritional regulation. We found that upon high fat diet (HFD), reduced hypothalamic polysialylation exacerbated the diet-induced obese phenotype in mice. Upon HFD, the histone acetyltransferase MOF was rapidly recruited on the St8sia4 polysialyltransferase-encoding gene. Mof silencing in the mediobasal hypothalamus of adult mice prevented…

Impact of a high fat high sucrose maternal diet on the progeny's olfactory system

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High-NaCl perception in drosophila melanogaster

Salt is a fundamental nutrient that is required for many physiological processes, including electrolyte homeostasis and neuronal activity. In mammals and Drosophila, the detection of NaCl induces two different behaviors: low-salt concentrations provide an attractive stimulus, whereas high-salt concentrations are avoided. We identified the gene called serrano (sano) as being expressed in the sensory organs of Drosophila larvae. A transgenic reporter line showed that sano was coexpressed with Gr66a in a subset of gustatory neurons in the terminal organ of third-instar larvae. The disruption of sano gene expression in gustatory neurons led to the specific loss of high-salt concentration avoida…

Centrifugal projections to the main olfactory bulb revealed by trans‐synaptic retrograde tracing in mice

A wide range of evidence indicates that olfactory perception is strongly involved in food intake. However, the polysynaptic circuitry linking the brain areas involved in feeding behavior to the olfactory regions is not well known. The aim of this article was to examine such circuits. Thus, we described, using hodological tools such as transsynaptic viruses (PRV152) transported in a retrograde manner, the long-distance indirect projections (two to three synapses) onto the main olfactory bulb (MOB). The ß-subunit of the cholera toxin which is a monosynaptic retrograde tracer was used as a control to be able to differentiate between direct and indirect projections. Our tracing experiments show…

Transient Receptor Potential Canonical 3 (TRPC3) Channels Are Required for Hypothalamic Glucose Detection and Energy Homeostasis

Fil: Chrétien, Chloé. University of Bourgogne Franche-Comté. Institut National de la Recherche Agronomique. Centre des Sciences du Goût et de l’Alimentation; France Fil: Fenech, Claire. University of Bourgogne Franche-Comté. Institut National de la Recherche Agronomique. Centre des Sciences du Goût et de l’Alimentation; France Fil: Liénard, Fabienne. University of Bourgogne Franche-Comté. Institut National de la Recherche Agronomique. Centre des Sciences du Goût et de l’Alimentation; France Fil: Grall, Sylvie. University of Bourgogne Franche-Comté. Institut National de la Recherche Agronomique. Centre des Sciences du Goût et de l’Alimentation; France Fil: Chevalier, Charlène. University of …

Impact of a maternal high fat high sucrose diet on progeny's olfactory system

International audience