Use of translational genomics to identify genes important for legume seed filling

International audience; Translational genomics, i.e., the transfer of genetic information from model species to cultivated crops, is on the brink of revolutionizing plant breeding. The recent publication of genomic sequences for several cultivated legumes is also accelerating this process. For pea, recent highthroughput RNA sequencing, and the prospect of a genome sequencing project, will further accelerate the transfer of information from the Medicago truncatula model to the cultivated crop. We have been using genomics approaches with Medicago as a tool to identify key genes determining seed yield and composition in closely related legumes. Analyses of the proteome and transcriptome of the…

Endosperm cintribution to Medicago truncatula seed development : characterization of a DOF transcription factor expressed in chalazal endosperm

In the current context, which necessitates a reduction in inputs in crop systems and boosting of production of plant proteins to reduce France’s dependency on feed imports,, growing legumes represents an alternative. Grain legumes are major sources of proteins for animal and human nutrition. In the UMR1347 Agroécologie, the objectives of the study group "déterminismes Génétiques et Environnementaux de l’Adaptation des Plantes à des Systèmes de culture Innovants" (GEAPSI) are to promote legume cultivation and adaptation to environmental stresses, via multidisciplinary approaches (genetics, ecophysiology, molecular physiology). This thesis project was carried out in the "Étude des Mécanismes …

Regulation of legume seed size by an endosperm-expressed transcription factor

BAP Pôle GEAPSI; International audience; There are numerous reports of transcription factors (TFs) which are implicated in the control of seed size and seed composition. We have identified, using a platform of TF sequences derived from the Medicago truncatula genome sequence, a class of TFs specifically expressed during the seed filling stage. One such TF, DASH, was shown to be confined to the developing endosperm. We investigated the role played by DASH through analysis of mutant alleles. These give rise to seed-lethal or near-lethal phenotypes, with degeneration of the endosperm and arrested embryo development. The relation of this phenotype to seed auxin action was investigated.

The roles of the embryo-surrounding tissues in regulating Medicago truncatula seed filling

National audience

Contribution de l'albumen au développement de la graine chez Medicago truncatula : caractérisation d'un facteur de transcription de type DOF exprimé dans l'albumen chalazal

In the current context, which necessitates a reduction in inputs in crop systems and boosting of production of plant proteins to reduce France’s dependency on feed imports,, growing legumes represents an alternative. Grain legumes are major sources of proteins for animal and human nutrition. In the UMR1347 Agroécologie, the objectives of the study group "déterminismes Génétiques et Environnementaux de l’Adaptation des Plantes à des Systèmes de culture Innovants" (GEAPSI) are to promote legume cultivation and adaptation to environmental stresses, via multidisciplinary approaches (genetics, ecophysiology, molecular physiology). This thesis project was carried out in the "Étude des Mécanismes …

Un facteur de transcription impliqué dans le développement de la graine chez Medicago truncatula