0000000000866867

AUTHOR

Feliciano Protasi

showing 2 related works from this author

Impaired Binding to Junctophilin-2 and Nanostructural Alteration in CPVT Mutation

2021

Rationale: Catecholaminergic polymorphic ventricular tachycardia (CPVT) is a rare disease, manifested by syncope or sudden death in children or young adults under stress conditions. Mutations in the Ca 2+ release channel/RyR2 (type 2 ryanodine receptor) gene account for about 60% of the identified mutations. Recently, we found and described a mutation in RyR2 N-terminal domain, RyR2 R420Q . Objective: To determine the arrhythmogenic mechanisms of this mutation. Methods and Results: Ventricular tachycardias under stress conditions were observed in both patients with catecholaminergic polymorphic ventricular tachycardia and knock-in mice. During action potential recording (by patch-clamp in …

Ile de francePhysiologyCPVT030204 cardiovascular system & hematologyArticle03 medical and health sciences0302 clinical medicineaction potential[SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular systemPolitical sciencejunctophilinryanodine receptormedia_common.cataloged_instanceHumansEuropean union610 Medicine & health030304 developmental biologymedia_common0303 health sciencescalciumRyanodine Receptor Calcium Release ChannelRyR2musculoskeletal systemSarcoplasmic ReticulumDeath Sudden Cardiaccalcium induced calcium releaseGain of Function Mutationcardiomyocyte calcium handlingcardiovascular systemventricular tachycardiamutationCardiology and Cardiovascular MedicineHumanities
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A perspective analysis: microRNAs, glucose metabolism, and drug resistance in colon cancer stem cells

2021

Metabolism sustains the stemness of Cancer Stem Cells (CSCs), affecting, in turn, tumor heterogeneity, metastatic potential, and therapy resistance. Therefore, it is appealing to target CSCs metabolism as a new therapeutic approach. Consequently, we paid considerable attention to the anti-apoptotic microRNA miR-483-3p, that we reported being regulated by glucose metabolism in liver cancer cells. We investigated the therapeutic potential of targeting miR-483-3p by using the anti-glucose metabolism 2-deoxyglucose (2-DG) molecule in tumor Xenograft mouse model originating from two different Colon-Cancer Stem Cell lines (CCSC lines). We show that 2-DG treatment does not affect CCSCs during tumo…

cancer stem cellCancer ResearchColorectal cancerDrug resistanceCarbohydrate metabolismText miningCell Line TumormicroRNAHumansMedicineMolecular Biologybusiness.industryPerspective (graphical)medicine.disease2-DGGene Expression Regulation NeoplasticMicroRNAsGlucosecolon cancerDrug Resistance NeoplasmColonic NeoplasmsNeoplastic Stem CellsCancer researchMolecular MedicineSettore MED/46 - Scienze Tecniche Di Medicina Di LaboratorioStem cellbusinessmetabolismCancer Gene Therapy
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