Diversity Arrays Technology (DArT) for Pan-genomic Evolutionary Studies of Non-model Organisms
BackgroundHigh-throughput tools for pan-genomic study, especially the DNA microarray platform, have sparked a remarkable increase in data production and enabled a shift in the scale at which biological investigation is possible. The use of microarrays to examine evolutionary relationships and processes, however, is predominantly restricted to model or near-model organisms.Methodology/principal findingsThis study explores the utility of Diversity Arrays Technology (DArT) in evolutionary studies of non-model organisms. DArT is a hybridization-based genotyping method that uses microarray technology to identify and type DNA polymorphism. Theoretically applicable to any organism (even one for wh…
Taxonomic uncertainty and a continental conundrum:Polypodium macaronesicumreassessed
Data from the chloroplast trnL-F region are used to test the delimitation of putative diploid species in the Polypodium cambricum aggregate. In particular, we investigate the distinctiveness of the Macaronesian Polypodium macaronesicum, P. azoricum and the continental P. cambricum, investigate molecular diversity patterns within Macaronesia and establish the identity of putative P. macaronesicum material collected from an area in southern Spain, where it co-occurs with other Macaronesian elements. The analysis supports the distinction of Macaronesian and continental plants, with accessions from Macaronesia resolved as monophyletic. Greater haplotype diversity was found in Macaronesia than o…
Comment on the letter of the Society of Vertebrate Paleontology (SVP) dated April 21, 2020 regarding “Fossils from conflict zones and reproducibility of fossil-based scientific data”: Myanmar amber
Motivation for this comment Recently, the Society of Vertebrate Paleontology (SVP) has sent around a letter, dated 21st April, 2020 to more than 300 palaeontological journals, signed by the President, Vice President and a former President of the society (Rayfield et al. 2020). The signatories of this letter request significant changes to the common practices in palaeontology. With our present, multi-authored comment, we aim to argue why these suggestions will not lead to improvement of both practice and ethics of palaeontological research but, conversely, hamper its further development. Although we disagree with most contents of the SVP letter, we appreciate this initiative to discuss scien…