0000000000870855

AUTHOR

Henning Thomasen

showing 2 related works from this author

The Effect of Long-Term Storage on the Biological and Histological Properties of Cryopreserved Amniotic Membrane

2011

Cryopreserved amniotic membrane (AM) is widely used in ophthalmology because of its anti-angiogenic, anti-inflammatory, and wound healing promoting capabilities. A common method to conserve the tissue is the storage in cryo-medium containing 50% glycerol at -80°C. The aim of this study was to examine the influence of storage time on the sterility as well as the histological and biological properties of cryopreserved AM.Amniotic membrane from different donors was stored in cell culture media containing 50% glycerol for different time periods, on average 4 months (group 1), 15 months (group 2), and 24 months (group 3), at -80°C. Samples of the tissue and cryo-medium were examined for bacteria…

Time FactorsSterilityBlotting WesternOrgan Preservation SolutionsMedizinTissue BanksBiologyCryopreservationAndrologyCellular and Molecular Neurosciencechemistry.chemical_compoundGlycerolHumansAmnionPolyacrylamide gel electrophoresisCryopreservationExtracellular Matrix ProteinsBacteriaBiological DressingsFungiOrgan PreservationSensory SystemsCulture MediaBlotOphthalmologyMembranechemistryTissue bankImmunologyCytokinesElectrophoresis Polyacrylamide GelWound healingBiomarkersCurrent Eye Research
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SARS-CoV-2: Impact on, Risk Assessment and Countermeasures in German Eye Banks.

2020

Introduction Since the beginning of the COVID-19 pandemic there has been some debate regarding the risk of transmission through tissue transplantation and tissue banking processes. Aim of the study To analyze the changes that SARS-CoV-2 has caused regarding the harvesting of corneal donor tissue and eye bank activities in Germany. Methods A questionnaire was provided to 26 eye banks in Germany, consisting of questions about adaptations made in the screening of potential donors and the harvesting of corneal tissue following the pandemic spread of SARS-CoV-2. Results Eighteen eye banks actively reduced recruitment of donors and two banks ceased all activity. Additional diagnostic screening wa…

Face shieldmedicine.medical_specialtybusiness.product_categoryTissue and Organ ProcurementSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)MedizinEye BanksRisk AssessmentCorneal DiseasesCorneal Transplantation03 medical and health sciencesCellular and Molecular Neuroscience0302 clinical medicineCorneaGermanySurveys and QuestionnairesPandemicmedicineDisease Transmission InfectiousHumansIntensive care medicinebusiness.industrySARS-CoV-2COVID-19Eye bankSensory SystemsTissue DonorsTransplantationOphthalmologymedicine.anatomical_structureMedical CountermeasuresDonationPractice Guidelines as TopicQuarantine030221 ophthalmology & optometryTissue and Organ Harvestingsense organsRisk assessmentbusiness030217 neurology & neurosurgeryCurrent eye research
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