0000000000876298
AUTHOR
Sébastien Carrère
showing 3 related works from this author
A transcriptomics-based approach enables the first identification of candidate genes involved in non-target-site-based resistance to herbicides in a …
2013
International audience; Herbicides are the most effective way to control weeds. Yet, the evolution of resistance in many weed species is a considerable threat to herbicide efficacy. Non-target-site-based resistance (NTSR) is the most threatening facet of herbicide resistance. NTSR is widespread, involves a variety of mechanisms, and can confer unpredictable resistance to herbicides, regardless of the herbicide mode of action. Because NTSR is endowed by differences in the expression of a number of genes in resistant and sensitive weed plants, few genes governing NTSR are currently known. This renders NTSR diagnosis and management tricky. The rise of next-generation sequencing technologies no…
Identification de mécanismes de résistance non liée à la cible grâce à la transcriptomique
2013
We have witnessed a dramatic increase in the number of weeds showing herbicide resistance during the past three decades. The potentially most dangerous mechanisms of resistance endows Non-Target-Site based Resistance (NTSR): NTSR is polygenic, unpredictable and can confer cross-resistance to herbicides with different modes of action. High throughput sequencing methods, like RNA sequencing (RNA-seq), are particularly effective to identify genes differentially expressed between two experimental modalities. Therefore, RNA sequencing was implemented to identify genes involved in NTSR to clodinafop (an ACCase inhibitor) in black-grass. Black-grass transcriptome was sequenced from 4 sensitive pla…
Diagnosis of non target site based resistance within rye grass(Lolium sp): development of quick and reliable tests
2013
Non target site resistance (NTSR) is the major threat for effective herbicide-based control of grass weeds. NTSR, still largely unknown, is endowed by the regulation of a subset of the genes involved in the response of the weed to herbicide treatment. This study aims at identifying NTSR genes in rye-grass to develop resistance diagnosis tests. The transcriptome of plants resistant or sensitive to ALS inhibitors collected before and after herbicide application was sequenced using a deep sequencing technology. We identified genes differentially expressed between resistant and sensitive plants. We developed a RT-qPCR test to measure gene expression levels in individual plants from several popu…