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RESEARCH PRODUCT

Identification de mécanismes de résistance non liée à la cible grâce à la transcriptomique

subject

description

We have witnessed a dramatic increase in the number of weeds showing herbicide resistance during the past three decades. The potentially most dangerous mechanisms of resistance endows Non-Target-Site based Resistance (NTSR): NTSR is polygenic, unpredictable and can confer cross-resistance to herbicides with different modes of action. High throughput sequencing methods, like RNA sequencing (RNA-seq), are particularly effective to identify genes differentially expressed between two experimental modalities. Therefore, RNA sequencing was implemented to identify genes involved in NTSR to clodinafop (an ACCase inhibitor) in black-grass. Black-grass transcriptome was sequenced from 4 sensitive plants and 4 clodinafop-resistant plants from the same F1 population, collected at different time-points before and after herbicide treatment. A black-grass transcriptome database was established that contains qualitative data (sequence of each gene and associated protein) and quantitative data (gene expression level of every gene during the time-course, in sensitive and in resistant plants). The expression of candidate NTSR genes was subsequently verified by quantitative RT-PCR.