0000000000877782

AUTHOR

Olgerts Marga

showing 2 related works from this author

Characterisation of rpsL, rrs and embB mutations associated with streptomycin and ethambutol resistance in Mycobacterium tuberculosis.

2003

In order to characterise molecular mechanisms of first-line drug resistance in Mycobacterium tuberculosis and to evaluate the use of molecular markers of resistance (gene point mutations), we analysed 66 multi-drug-resistant (MDR) isolates from Latvian tuberculosis patients. They were all resistant to rifampin (RIF), isoniazid (INH) and streptomycin (SM), and 33 were resistant to ethambutol (EMB). Enzymatic digestion by MboII and nucleotide sequencing of the rpsL gene fragment detected a single nucleotide substitution K43R in 40 (61%) of the 66 SM-resistant M. tuberculosis isolates. Of the other 26 SM-resistant isolates, 16 (24%) had mutations at positions 513A--C and 516C--T of the rrs gen…

DNA BacterialRibosomal ProteinsDrug resistanceGene mutationMicrobiologyPolymerase Chain ReactionMycobacterium tuberculosisAnti-Infective AgentsDrug Resistance Multiple BacterialRNA Ribosomal 16SmedicineHumansTuberculosisDeoxyribonucleases Type II Site-SpecificMolecular BiologyEthambutolPolymorphism Single-Stranded ConformationalAntibacterial agentGeneticsbiologyPoint mutationSingle-strand conformation polymorphismGeneral MedicineMycobacterium tuberculosisSequence Analysis DNAbiology.organism_classificationMolecular biologyStreptomycinStreptomycinEthambutolmedicine.drugResearch in microbiology
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Spectrum of pncA Mutations in Multidrug-Resistant Mycobacterium tuberculosis Isolates Obtained in Latvia

2004

Pyrazinamide (PZA) is an effective antituberculous agent ([1][1]) that becomes active when bacterial pyrazinamidase converts it to pyrazinoic acid, which is toxic to mycobacteria ([4][2]). In Mycobacerium tuberculosis , PZA resistance is associated with the loss of pyrazinamidase activity, mainly

TuberculosisAntitubercular AgentsAmidohydrolasesMicrobiologyMycobacterium tuberculosischemistry.chemical_compoundPyrazinoic acidDrug Resistance Multiple BacterialmedicineHumansTuberculosisPharmacology (medical)Multidrug-Resistant Mycobacterium tuberculosisCodonLetters to the EditorPharmacologybiologyReverse Transcriptase Polymerase Chain ReactionMycobacterium tuberculosisPyrazinamidebiology.organism_classificationmedicine.diseaseLatviaPyrazinamideVirologyInfectious DiseaseschemistryMutationPncAmedicine.drugAntimicrobial Agents and Chemotherapy
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