Automatic cell nuclei counting: a protocol to acquire images and to compare results between color and multispectral images

International audience; High-throughput screening in histology and analysis need a necessary automatic cell or nucleus counting. Current methods and systems based on grayscale or color images give results with counting errors. We suggest to use multispectral imaging (with more than three bands) rather than color one for nucleus counting. A traditional acquisition chains uses a source of white light and a CCD camera in addition to the optical microscope. To pass to a multispectral acquisition, we use a Programmable Light Source (PLS) in the place of the white light source. This PLS is capable of generating different wavelengths in the visible spectrum. So, one color image and four multispect…