0000000000894547
AUTHOR
Mar Villar-torres
MOESM9 of In vivo fluorescent cercariae reveal the entry portals of Cardiocephaloides longicollis (Rudolphi, 1819) Dubois, 1982 (Strigeidae) into the gilthead seabream Sparus aurata L.
Additional file 9: Table S9. Evaluation of the effect of dyes on cercarial infectivity and metacercarial encystment success.
Additional file 2: of Come rain or come shine: environmental effects on the infective stages of Sparicotyle chrysophrii, a key pathogen in Mediterranean aquaculture
Table S2. Larval longevity and behaviour of S. chrysophrii by replicate at each temperature. (DOCX 15 kb)
MOESM8 of In vivo fluorescent cercariae reveal the entry portals of Cardiocephaloides longicollis (Rudolphi, 1819) Dubois, 1982 (Strigeidae) into the gilthead seabream Sparus aurata L.
Additional file 8: Table S8. Evaluation of the effect of sub-regions of fish’s surface on cercarial density.
MOESM2 of In vivo fluorescent cercariae reveal the entry portals of Cardiocephaloides longicollis (Rudolphi, 1819) Dubois, 1982 (Strigeidae) into the gilthead seabream Sparus aurata L.
Additional file 2: Table S2. Evaluation of the effect of different NB concentrations on cercarial survival.
MOESM5 of In vivo fluorescent cercariae reveal the entry portals of Cardiocephaloides longicollis (Rudolphi, 1819) Dubois, 1982 (Strigeidae) into the gilthead seabream Sparus aurata L.
Additional file 5: Table S5. Evaluation of the effect of CFSE concentration treatment on cercarial activity.
MOESM1 of In vivo fluorescent cercariae reveal the entry portals of Cardiocephaloides longicollis (Rudolphi, 1819) Dubois, 1982 (Strigeidae) into the gilthead seabream Sparus aurata L.
Additional file 1: Table S1. Evaluation of the effect of fish’s flank on cercarial attachment success.
MOESM7 of In vivo fluorescent cercariae reveal the entry portals of Cardiocephaloides longicollis (Rudolphi, 1819) Dubois, 1982 (Strigeidae) into the gilthead seabream Sparus aurata L.
Additional file 7: Table S7. Evaluation of the effect of major regions of fish’s surface on cercarial density.
First Stranding Event of a Common Minke Whale Calf,Balaenoptera acutorostrataLacépède, 1804, Reported in Spanish Mediterranean Waters
1 Marine Zoology Unit, Cavanilles Institute of Biodiversity and Evolutionary Biology, University of Valencia, PO Box 22085, 46071 Valencia, Spain 2 Oceanografic, Veterinary Services, Parques Reunidos Valencia, Ciudad de las Artes y las Ciencias, C/ Eduardo Primo Yufera 1B, 46013 Valencia, Spain 3 VISAVET Center and Animal Health Department, Veterinary School, Complutense University of Madrid, Av Puerta del Hierro s/n, 28040 Madrid, Spain
MOESM4 of In vivo fluorescent cercariae reveal the entry portals of Cardiocephaloides longicollis (Rudolphi, 1819) Dubois, 1982 (Strigeidae) into the gilthead seabream Sparus aurata L.
Additional file 4: Table S4. Evaluation of the effect of CFSE different concentrations on cercarial survival.
Additional file 5: of Come rain or come shine: environmental effects on the infective stages of Sparicotyle chrysophrii, a key pathogen in Mediterranean aquaculture
Table S5. Parameters of embryonic development of S. chrysophrii by replicate at each light regime. (DOC 36 kb)
Additional file 8: of Come rain or come shine: environmental effects on the infective stages of Sparicotyle chrysophrii, a key pathogen in Mediterranean aquaculture
Table S8. Larval longevity and behaviour of S. chrysophrii by replicate at different salinities and temperatures. (DOCX 15 kb)
Additional file 6: of Come rain or come shine: environmental effects on the infective stages of Sparicotyle chrysophrii, a key pathogen in Mediterranean aquaculture
Table S6. Larval longevity and behaviour of S. chrysophrii by replicate at each light regime. (DOCX 13 kb)
Additional file 3: of Come rain or come shine: environmental effects on the infective stages of Sparicotyle chrysophrii, a key pathogen in Mediterranean aquaculture
Table S3. Parameters of embryonic development of S. chrysophrii by replicate at each pH level. (DOC 37 kb)
MOESM6 of In vivo fluorescent cercariae reveal the entry portals of Cardiocephaloides longicollis (Rudolphi, 1819) Dubois, 1982 (Strigeidae) into the gilthead seabream Sparus aurata L.
Additional file 6: Table S6. Evaluation of the effect of dyes on cercarial survival and activity.
Neither Diplectanum nor specific: a dramatic twist to the taxonomic framework of Diplectanum (Monogenea: Diplectanidae).
The taxonomy of the genus Diplectanum has been exclusively based on morphological features, with 28 nominal species parasitic on perciform fishes recognised. We used molecular data, to our knowledge for the first time, to evaluate the taxonomic framework of Diplectanum, infer the relationships amongst species attributed to this genus, re-assess the degree of host specificity and explore the population genetic structure of Diplectanum spp. parasitising Mediterranean sciaenids, which are potential target fish species for aquaculture diversification in the region. A minimum of 10 specimens of Diplectanum spp. were sequenced per host species (Argyrosomus regius, Sciaena umbra, Umbrina canariens…
Additional file 7: of Come rain or come shine: environmental effects on the infective stages of Sparicotyle chrysophrii, a key pathogen in Mediterranean aquaculture
Table S7. Parameters of embryonic development of S. chrysophrii by replicate at different salinities and temperatures. (DOC 45 kb)
Additional file 4: of Come rain or come shine: environmental effects on the infective stages of Sparicotyle chrysophrii, a key pathogen in Mediterranean aquaculture
Table S4. Larval longevity and behaviour of S. chrysophrii by replicate at each pH level. (DOCX 13 kb)
Additional file 1: of Come rain or come shine: environmental effects on the infective stages of Sparicotyle chrysophrii, a key pathogen in Mediterranean aquaculture
Table S1. Parameters of embryonic development of S. chrysophrii by replicate at each temperature. (DOCX 15 kb)
MOESM3 of In vivo fluorescent cercariae reveal the entry portals of Cardiocephaloides longicollis (Rudolphi, 1819) Dubois, 1982 (Strigeidae) into the gilthead seabream Sparus aurata L.
Additional file 3: Table S3. Evaluation of the effect of NB concentration treatment on cercarial activity.
MOESM10 of In vivo fluorescent cercariae reveal the entry portals of Cardiocephaloides longicollis (Rudolphi, 1819) Dubois, 1982 (Strigeidae) into the gilthead seabream Sparus aurata L.
Additional file 10: Table S10. Evaluation of the effect of fish’s head sub-regions on cercarial density.
Come rain or come shine: environmental effects on the infective stages of Sparicotyle chrysophrii, a key pathogen in Mediterranean aquaculture
Background Evidence concerning the environmental influence on monogenean transmission and infection processes is widely accepted, although only the effects of a limited number of abiotic factors on particular monogenean species have been explored. The current context of climate change calls for further research both on this subject, and also that concerning monogenean hosts, especially in aquaculture. Methods In this study, four experiments were used to assess the response of the infective stages of Sparicotyle chrysophrii, a pathogenic monogenean from gilthead sea bream (Sparus aurata) cultures in the Mediterranean, to variations of temperature (from 10 °C to 30 °C), pH (7.0 and 7.9), phot…
In vivo fluorescent cercariae reveal the entry portals of Cardiocephaloides longicollis (Rudolphi, 1819) Dubois, 1982 (Strigeidae) into the gilthead seabream Sparus aurata L.
Background Despite their complex life-cycles involving various types of hosts and free-living stages, digenean trematodes are becoming recurrent model systems. The infection and penetration strategy of the larval stages, i.e. cercariae, into the fish host is poorly understood and information regarding their entry portals is not well-known for most species. Cardiocephaloides longicollis (Rudolphi, 1819) Dubois, 1982 (Digenea, Strigeidae) uses the gilthead seabream (Sparus aurata L.), an important marine fish in Mediterranean aquaculture, as a second intermediate host, where they encyst in the brain as metacercariae. Labelling the cercariae with in vivo fluorescent dyes helped us to track the…