Implication of specific isoforms of p38 in termothollerance and in the response to different stresses in sea urchin embryo

MAPK activation is required for Paracentrotus lividus skeletogenesis

P38 MAPK expression during development and in stress response in sea urchin embryo

P38 MAPK plays a key role in skeletogenesis of Paracentrotus lividus embryo

Espressione basale dell’HSP70 inducibile in differenti linee staminali di topo.

Effect of cadmium on MDA-MB231 breast cancer cells

We demonstrated that treatment of estrogen receptor-negative (ER-) MDA-MB231 breast cancer cells with 5 M Cd for 96h resulted in an about 50% reduction of cell number

Nickel, lead, and cadmium induce differential cellular responses in sea urchin embryos by activating the synthesis of different HSP70s.

Treatment with heavy metals, such as nickel, lead or cadmium, elicits different cellular stress responses according to the metal used and the length of treatment. In Paracentrotus lividus embryos the inducible forms of HSP70 (HSP70/72) are different in molecular mass from the constitutively expressed HSP75, and they can be used as markers of cellular stress. Even a short treatment with each metal induces the synthesis of HSP70/72 which remain stable for at least 20 h and differ little in their isoelectric points. Continuous treatment from fertilization with nickel or lead produces late irregular pluteus embryos, with peak HSP70/72 synthesis at blastula followed by the arrest of synthesis by…

Implication of p38 MAPK in skeletogenesis of Paracentrotus lividus embryo

Different Kind of stress activate differnt isoforms of p38MAPK in sea urchin embryos

p38 MAPK activity during sea urchin embryo development (Paracentrotus lividus)

Effect of cadmium on MDA-MB231 breast cancer cells

P38 mapk activity during sea urchin embryo development

EFFECT OF CADMIUM ON IMMORTALIZED AND NEOPLASTIC EPITHELIAL CELLS OF THE HUMAN BREAST

Cadmium effect on gene expression by MDA-MB231 breast cancer cells: evidence for down-regulation of AEG-1

p38 MAPK activation is required for Paracentrotus lividus skeletogenesis

We investigated the p38 MAPK role during sea urchin, Paracentrotus lividus, development. We found that at the morula stage, before the onset of skeletogenesis, p38 MAPK shows a peak of activity, and we tested whether p38 MAPK activity has any effect on skeletogenesis. By immunohistochemistry on whole-mount embryos we show the preferential localization of the active p38 form both in the presumptive PMCs and bilateral spiculo- genesis centers in control embryos, and in the radialized supernumerary spiculogenesis centers induced by NiCl2 treatment. By using SB203580, a p38 MAPK specific inhibitor, we show that p38 activity is required both for the initial triradiate spicule rudiments formation…

Different kind of stress activate different isoforms of p38 MAPK in sea urchin embryos

Isoforme specifiche di p38 implicate nella termotolleranza e nella risposta a differenti stress nell'embrione di riccio di mare